Goat anti-PCK2/PEPCK-M Antibody | MyBioSource MBS420663 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-PCK2/PEPCK-M Antibody

catalog :

MBS420663

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420663

products type :

Antibody

products full name :

Goat anti-PCK2/PEPCK-M Antibody

products short name :

PCK2/PEPCK-M

products name syn :

PCK2; PEPCK-M; phosphoenolpyruvate carboxykinase 2 (mitochondrial); HGNC: 8725; PEPCK; PEPCK2; PEP carboxykinase; mitochondrial phosphoenolpyruvate carboxykinase 2; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; PCK2 antibody; PEPCK-M antibody; phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody; HGNC: 8725 antibody; PEPCK antibody; PEPCK2 antibody; PEP carboxykinase antibody; mitochondrial phosphoenolpyruvate carboxykinase 2 antibody; phosphoenolpyruvate carboxylase antibody; phosphopyruvate carboxylase antibody; PCK2 / PEPCK-M

other names :

phosphoenolpyruvate carboxykinase; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxykinase 2, mitochondrial

products gene name :

PCK2

products gene name syn :

PEPCK-M

other gene names :

PCK2; PCK2; PEPCK; PEPCK2; PEPCK-M; PEPCK2; PEPCK-M

uniprot entry name :

PCKGM_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Mouse, Rat, Guinea Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

sequence length :

640

sequence :

KPWKPGDKEPCAH

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 75kDa band observed in Human Liver lysates (calculated MW of 70.7kDa according to NP_004554.2). Recommended concentration: 2-4ug/ml. Confirmation plus additional tested rodent species was recently puiblished: PMID: 23466304.

other info1 :

Immunogen: Peptide with sequence KPWKPGDKEPCAH, from the internal region of the protein sequence according to NP_004554.2. Epitope: Internal region

other info2 :

Note: This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1.

ncbi gi num :

66346721

ncbi acc num :

NP_004554.2

ncbi gb acc num :

NM_004563.2

ncbi mol weight :

47,564 Da

ncbi pathways :

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); DNA Damage Response (only ATM Dependent) Pathway (198827); FoxO Signaling Pathway (921162); Glucagon Signaling Pathway (1144996)

ncbi summary :

This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]

uniprot summary :

PCK2: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Monomer. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Lyase; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.1.1.32; Mitochondrial. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: mitochondrial matrix; mitochondrion. Molecular Function: GTP binding; metal ion binding; phosphoenolpyruvate carboxykinase (GTP) activity; phosphoenolpyruvate carboxykinase activity; protein binding. Biological Process: gluconeogenesis. Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Mitochondrial

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage