catalog number :
MBS420662
products full name :
Goat anti-MURF2/TRIM55 Antibody
products short name :
MURF2/TRIM55
products name syn :
TRIM55; RNF29; MURF-2; ring finger protein 29; for muscle specific ring finger 2; tripartite motif-containing 55; muscle specific ring finger 2; TRIM55 antibody; RNF29 antibody; MURF-2 antibody; ring finger protein 29 antibody; for muscle specific ring finger 2 antibody; tripartite motif-containing 55 antibody; muscle specific ring finger 2 antibody; MURF2 / TRIM55
other names :
tripartite motif-containing protein 55 isoform 1; Tripartite motif-containing protein 55; tripartite motif-containing protein 55; tripartite motif containing 55; Muscle-specific RING finger protein 2; MuRF-2; MuRF2; RING finger protein 29
products gene name :
TRIM55
products gene name syn :
MURF2
other gene names :
TRIM55; TRIM55; RNF29; muRF2; MURF-2; MURF2; RNF29; MuRF-2; MuRF2
uniprot entry name :
TRI55_HUMAN
reactivity :
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 85kDa band observed in Human and Mouse Heart lysates (calculated MW of 60.5kDa according to NP_908973.1). The observed molecular weight corresponds to earlier findings in literature with the same antibody (Willis et al, Circ Res.2007 Mar 2;100(4): 456-9. PMID: 17272810). Recommended concentration: 0.1-0.3ug/ml.
other info1 :
Immunogen: Peptide with sequence C-HIFSFSWLNSLNE, from the C Terminus of the protein sequence according to NP_908973.1; NP_908974.1; NP_908975.1. Epitope: C Terminus
other info2 :
Note: This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp. ).
ncbi acc num :
NP_908973.1
ncbi gb acc num :
NM_184085.1
ncbi mol weight :
26,841 Da
ncbi summary :
The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein associates transiently with microtubules, myosin, and titin during muscle sarcomere assembly. It may act as a transient adaptor and plays a regulatory role in the assembly of sarcomeres. Four alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
uniprot summary :
TRIM55: May regulate gene expression and protein turnover in muscle cells. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase. Chromosomal Location of Human Ortholog: 8q13.1. Cellular Component: cytoplasm; microtubule; nucleus. Molecular Function: identical protein binding; protein binding; signal transducer activity; zinc ion binding. Biological Process: signal transduction