catalog number :
MBS420654
products full name :
Goat anti-Npap60 / Nup50 Antibody
products short name :
Npap60/Nup50
products name syn :
NUP50; NPAP60; NPAP60L; MGC39961; DKFZP564A043; nucleoporin 50kDa; nuclear pore-associated protein 60L; CTA-268H5.7; OTTHUMP00000199530; NUP50; NPAP60; NPAP60L; MGC39961; DKFZP564A043; nucleoporin 50kDa; nuclear pore-associated protein 60L; CTA-268H5.7; OTTHUMP00000199530
other names :
nuclear pore complex protein Nup50 isoform b; Nuclear pore complex protein Nup50; nuclear pore complex protein Nup50; nucleoporin 50kD; nucleoporin Nup50; 50 kDa nucleoporin; nuclear pore-associated protein 60L; nucleoporin 50kDa; 50 kDa nucleoporin; Nuclear pore-associated protein 60 kDa-like; Nucleoporin Nup50
products gene name :
NUP50
other gene names :
NUP50; NUP50; NPAP60; NPAP60L; NPAP60L
uniprot entry name :
NUP50_HUMAN
reactivity :
Human, Mouse, Rat, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
app notes :
Immunohistochemistry: In paraffin embedded Human Uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8ug/ml. Western Blot: Approx 60kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-ELHKILLEKKDA, from the C Terminus of the protein sequence according to NP_009103.2; NP_705931.1. Corresponding Immunizing Peptide: Catalog #MBS425945
other info2 :
Important Note: This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1).
products references :
Shitashige M, Satow R, Honda K, Ono M, Hirohashi S, Yamada T.; Regulation of Wnt signaling by the nuclear pore complex.; Gastroenterology. 2008 Jun; 134(7):1961-71, 1971.e1-4.; Pubmed 18439914
ncbi acc num :
NP_009103.2
ncbi gb acc num :
NM_007172.3
ncbi mol weight :
46,863 Da
ncbi pathways :
Antiviral Mechanism By IFN-stimulated Genes Pathway 530760!!Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Cytokine Signaling In Immune System Pathway 366171!!Disease Pathway 530764!!Export Of Viral Ribonucleoproteins From Nucleus Pathway 106080!!Gene Expression Pathway 105937!!Glucose Transport Pathway 106200!!Glycogen Storage Diseases Pathway 980468!!HIV Infection Pathway 105983
ncbi summary :
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]