catalog number :
MBS420653
products full name :
Goat anti-FOXP3 / SCURFIN Antibody
products short name :
FOXP3/SCURFIN
products name syn :
FOXP3; SCURFIN; forkhead box P3; JM2; AIID; IPEX; PIDX; XPID; DIETER; JM2 protein; immunodeficiency; polyendocrinopathy; enteropathy; X-linked; immune dysregulation; polyendocrinopathy; enteropathy; X-linked; MGC141961; MGC141963; FOXP3delta7; scurfin; FOXP3; SCURFIN; forkhead box P3; JM2; AIID; IPEX; PIDX; XPID; DIETER; JM2 protein; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; FOXP3delta7; scurfin
other names :
forkhead box protein P3 isoform a; Forkhead box protein P3; forkhead box protein P3; scurfin; FOXP3delta7; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; forkhead box P3; Scurfin
products gene name :
FOXP3
other gene names :
FOXP3; FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER; IPEX
uniprot entry name :
FOXP3_HUMAN
reactivity :
Human, Dog, Pig
sequence :
SQRPSRCSNPTPGP
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Chromatin immunoprecipitation (ChIP)
app notes :
Immunohistochemistry: In paraffin embedded Human Tonsil with stained nuclei of some cells in the interfollicular area. Recommended concentration, 0.3-1 ug/ml. Western Blot: This antibody has been successfully used in WB on human T regulatory cells. Peptide ELISA: antibody detection limit dilution 1:8000. Chromatin immunoprecipitation: This antibody has been successfully used in ChIP on activated human T cells.
Immunocytochemistry: CD25-sorted (Treg) human blood cells with nuclear speckled staining. Recommended concentration 2-4ug/ml for overnight staining.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2; NP_001107849.1. Corresponding Immunizing Peptide: Catalog #MBS426663
other info2 :
Important Note: This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)
ncbi acc num :
NP_054728.2
ncbi gb acc num :
NM_014009.3
ncbi mol weight :
44,407 Da
ncbi pathways :
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!IL2 Signaling Events Mediated By STAT5 Pathway 137988!!Inflammatory Bowel Disease (IBD) Pathway 842771!!Inflammatory Bowel Disease (IBD) Pathway 842797
ncbi summary :
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]