antibody

Goat anti-ROBO3 / RIG1 (internal) Antibody

MBS420646

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, dog, cow

western blot, enzyme immunoassay

Antibody

Goat anti-ROBO3 / RIG1 (internal) Antibody

ROBO3/RIG1

ROBO3; RIG1; roundabout; axon guidance receptor; homolog 3 (Drosophila); FLJ21044; HGPPS; RBIG1; retinoblastoma inhibiting gene 1; roundabout; axon guidance receptor; homolog 3; Goat Anti-ROBO3 (internal); ROBO3; RIG1; roundabout, axon guidance receptor, homolog 3 (Drosophila); FLJ21044; HGPPS; RBIG1; retinoblastoma inhibiting gene 1; roundabout, axon guidance receptor, homolog 3

roundabout homolog 3; Roundabout homolog 3; roundabout homolog 3; roundabout-like protein 3; retinoblastoma inhibiting gene 1; roundabout, axon guidance receptor, homolog 3 (Drosophila); Roundabout-like protein 3

ROBO3

ROBO3; ROBO3; HGPS; RIG1; HGPPS; RBIG1

ROBO3_HUMAN

Polyclonal

Goat

Human, Dog, Pig, Cow

1386

RRKQRKELSHYT

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Western Blot: Approx 150kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 148kDa according to NP_071765.2). Recommended concentration: 0.5-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.

Epitope: Internal Region. Immunogen: Peptide with sequence C-RRKQRKELSHYT, from the internal region of the protein sequence according to NP_071765.2.

Corresponding Immunizing Peptide: Catalog #MBS425619

48476182

NP_071765.2

NM_022370.3

110,676 Da

Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!Axon Guidance Pathway 105688!!Developmental Biology Pathway 477129!!Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway 119528!!Signaling By Robo Receptor Pathway 119527

This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009]

0.1 mg

290 USD