catalog number :
MBS420645
products full name :
Goat anti-RAD9A Antibody
products short name :
RAD9A
products name syn :
RAD9A; RAD9 homolog A (S. pombe); RAD9; RAD9 (S. pombe) homolog; RAD9 homolog; RAD9 homolog (S. pombe); cell cycle checkpoint control protein; RAD9A; RAD9 homolog A (S. pombe); RAD9; RAD9 (S. pombe) homolog; RAD9 homolog; RAD9 homolog (S. pombe); cell cycle checkpoint control protein
other names :
cell cycle checkpoint control protein RAD9A isoform 1; Cell cycle checkpoint control protein RAD9A; cell cycle checkpoint control protein RAD9A; hRAD9; DNA repair exonuclease rad9 homolog A; RAD9 homolog A (S. pombe); DNA repair exonuclease rad9 homolog A
products gene name :
RAD9A
other gene names :
RAD9A; RAD9A; RAD9; hRAD9
uniprot entry name :
RAD9A_HUMAN
reactivity :
Human, Mouse, Dog
sequence :
QGPSPVLAEDSEGE
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), ELISA (EIA)
app notes :
Western Blot: Approx 70kDa band observed in lysates of cell lines A431 and Daudi (calculated MW of 42.5kDa according to NP_004575.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Volkmer et al, J Biol Chem. 1999 Jan 8;274(2):567-70.; PMID: 9872989). Recommended concentration: 0.3-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant RAD9A captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-QGPSPVLAEDSEGE, from the C Terminus of the protein sequence according to NP_004575.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425149
ncbi acc num :
NP_004575.1
ncbi gb acc num :
NM_004584.2
ncbi mol weight :
42,547 Da
ncbi pathways :
Activation Of ATR In Response To Replication Stress Pathway 105754!!Androgen Receptor Signaling Pathway 198806!!Cell Cycle Pathway 530733!!Cell Cycle Checkpoints Pathway 105739!!DNA Damage Response Pathway 198788!!G2/M Checkpoints Pathway 105750!!Regulation Of Telomerase Pathway 137987
ncbi summary :
This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]