catalog number :
MBS420625
products full name :
Goat anti-RNF34 / RFI (internal) Antibody
products short name :
RNF34/RFI
products name syn :
RNF34; ring finger protein 34; FLJ21786; RFI; RIF; RIFF; FYVE-RING finger protein MOMO; RING finger protein RIFF; hRFI; Goat Anti-RNF34 / RFI (N Terminus); RNF34; ring finger protein 34; FLJ21786; RFI; RIF; RIFF; FYVE-RING finger protein MOMO; RING finger protein RIFF; hRFI
other names :
E3 ubiquitin-protein ligase RNF34 isoform 1; E3 ubiquitin-protein ligase RNF34; E3 ubiquitin-protein ligase RNF34; caspase regulator CARP1; RING finger protein RIFF; FYVE-RING finger protein MOMO; caspases-8 and -10-associated RING finger protein 1; human RING finger homologous to inhibitor of apoptosis protein; ring finger protein 34, E3 ubiquitin protein ligase; Caspase regulator CARP1; FYVE-RING finger protein Momo; Human RING finger homologous to inhibitor of apoptosis protein; RING finger protein 34; RING finger protein RIFF
products gene name :
RNF34
other gene names :
RNF34; RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1
uniprot entry name :
RNF34_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA)
app notes :
Western Blot: Preliminary experiments gave an approx 35kDa band in Human Lymph Node, Ovary and Testis lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.7kDa according to NP_919247.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. Peptide ELISA: antibody detection limit dilution 1:64000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-RLYKENEENQKSY, from the internal region of the protein sequence according to NP_919247.1; NP_079402.2. Corresponding Immunizing Peptide: Catalog #MBS425356
other info2 :
Important Note: This antibody is expected to recognize both reported isoforms (NP_919247.1; NP_079402.2).
ncbi acc num :
NP_919247.1
ncbi gb acc num :
NM_194271.2
ncbi mol weight :
41,797 Da
ncbi pathways :
Adaptive Immune System Pathway 366160!!Antigen Processing: Ubiquitination Proteasome Degradation Pathway 366162!!Class I MHC Mediated Antigen Processing Presentation Pathway 366161!!Immune System Pathway 106386
ncbi summary :
The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]