catalog number :
MBS420614
products full name :
Goat anti-MAD2L1 Antibody
products short name :
MAD2L1
products name syn :
MAD2L1; MAD2 mitotic arrest deficient-like 1 (yeast); MAD2; HSMAD2; mitotic arrest deficient; yeast; homolog-like 1; MAD2 (mitotic arrest deficient; yeast; homolog)-like 1; MAD2-like 1; MAD2-like protein 1; MAD2L1; MAD2 mitotic arrest deficient-like 1 (yeast); MAD2; HSMAD2; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2-like 1; MAD2-like protein 1
other names :
mitotic spindle assembly checkpoint protein MAD2A; Mitotic spindle assembly checkpoint protein MAD2A; mitotic spindle assembly checkpoint protein MAD2A; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2 mitotic arrest deficient-like 1 (yeast); Mitotic arrest deficient 2-like protein 1; MAD2-like protein 1
products gene name :
MAD2L1
other gene names :
MAD2L1; MAD2L1; MAD2; HSMAD2; MAD2; HsMAD2; MAD2-like protein 1
uniprot entry name :
MD2L1_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Pig, Cow
sequence :
KVNSMVAYKIPVND
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 24kDa band observed in lysates of cell line HEK293 (calculated MW of 23.5kDa according to NP_002349.1). Recommended concentration: 2-4ug/ml.
An additional band of unknown identity was also consistently observed at approx. 29kDa. This band was successfully blocked by incubation with the immunizing peptide. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-KVNSMVAYKIPVND, from the C Terminus of the protein sequence according to NP_002349.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS426254
ncbi acc num :
NP_002349.1
ncbi gb acc num :
NM_002358.3
ncbi mol weight :
10,335 Da
ncbi pathways :
APC/C-mediated Degradation Of Cell Cycle Proteins Pathway 105825!!APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105831!!Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105829!!Amplification Of Signal From Unattached Kinetochores Via A MAD2 Inhibitory Signal Pathway 105761!!Amplification Of Signal From The Kinetochores Pathway 105760!!Cell Cycle Pathway 530733!!Cell Cycle Checkpoints Pathway 105739!!Cell Cycle, Mitotic Pathway 105765!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054
ncbi summary :
MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]