Goat anti-SIRT4 Antibody | MyBioSource MBS420605 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-SIRT4 Antibody

catalog :

MBS420605

quantity :

0.1 mg

price :

305 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

(1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

image 2 :

(2.5 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

product information

catalog number :

MBS420605

products type :

Antibody

products full name :

Goat anti-SIRT4 Antibody

products short name :

[SIRT4]

products name syn :

[SIRT4; SIR2L4; sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae); sirtuin silent mating type information regulation 2 homolog 4 (S. cerevisiae); MGC130046; MGC130047; MGC57437; SIR2L4; sirtuin 4; sir2-like 4; sirtuin 4; sirtu; SIRT4 antibody; SIR2L4 antibody; sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) antibody; sirtuin silent mating type information regulation 2 homolog 4 (S. cerevisiae) antibody; MGC130046 antibody; MGC130047 antibody; MGC57437 antibody; SIR2L4 antibody; sirtuin 4 antibody; sir2-like 4 antibody; sirtuin 4 antibody; sirtuin type 4 antibody]

other names :

[NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; sirtuin 4; NAD-dependent ADP-ribosyltransferase sirtuin-4]

products gene name :

[SIRT4]

other gene names :

[SIRT4; SIRT4; SIR2L4]

uniprot entry name :

SIR4_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human. Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

sequence length :

314

sequence :

SRCGELLPLIDPC

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

storage stability :

Aliquot and store at -20°C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Western blot: Approx. 38kDa band observed in Human Kidney lysates (calculated MW of 35.2kDa according to NP_036372.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. IHC: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration: 2-3ug/ml.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Peptide with sequence SRCGELLPLIDPC, from the C Terminus of the protein sequence according to NP_036372.1.

ncbi gi num :

6912662

ncbi acc num :

NP_036372.1

ncbi gb acc num :

NM_012240.2

ncbi pathways :

Signaling Events Mediated By HDAC Class I Pathway (137997)

ncbi summary :

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]

uniprot summary :

SIRT4: NAD-dependent protein ADP-ribosyl transferase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1. Inhibits GLUD1 enzyme activity. Down- regulates insulin secretion. Has no detectable protein deacetylase activity. Belongs to the sirtuin family. Class II subfamily. Protein type: Tumor suppressor; EC 2.4.2.-; EC 3.5.1.-; Mitochondrial; Deacetylase. Chromosomal Location of Human Ortholog: 12q. Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion. Molecular Function: biotinidase activity; metal ion binding; NAD+ ADP-ribosyltransferase activity; protein binding. Biological Process: chromatin silencing; glutamine metabolic process; negative regulation of fatty acid oxidation; negative regulation of insulin secretion; positive regulation of lipid biosynthetic process; protein amino acid ADP-ribosylation; regulation of glutamine family amino acid metabolic process; response to DNA damage stimulus

size1 :

0.1 mg

price1 :

305 USD

more info or order :

MyBioSource product webpage