antibody

Goat anti-MDA5/Ifih1 (mouse) Antibody

MBS420601

0.1 mg

225 USD

polyclonal

goat

nonconjugated

ELISA, enzyme immunoassay

Antibody

Goat anti-MDA5/Ifih1 (mouse) Antibody

MDA5/Ifih1

Ifih1; interferon induced with helicase C domain 1; 9130009C22Rik; Helicard; Hlcd; MDA-5; MDA5; MGC90959; melanoma differentiation associated protein-5; Ifih1 antibody; interferon induced with helicase C domain 1 antibody; 9130009C22Rik antibody; Helicard antibody; Hlcd antibody; MDA-5 antibody; MDA5 antibody; MGC90959 antibody; melanoma differentiation associated protein-5 antibody; MDA5 / Ifih1 (mouse)

interferon induced with helicase C domain 1; Interferon-induced helicase C domain-containing protein 1; interferon-induced helicase C domain-containing protein 1; interferon induced with helicase C domain 1; Helicase with 2 CARD domains; Helicard; Interferon induced with helicase C domain protein 1; Melanoma differentiation-associated protein 5; MDA-5; RIG-I-like receptor 2; RLR-2

Ifih1

MDA5

Ifih1; Ifih1; Hlcd; MDA5; RLR-2; Helicard; 9130009C22Rik; Helicard; MDA-5; RLR-2

IFIH1_MOUSE

Polyclonal

Goat

Expected from sequence similarity: Mouse

1025

KQKLRNNDLKKQN

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA)

Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Preliminary experiments gave an approx 80kDa band in Mouse Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116kDa according to NP_082111.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.

Immunogen: Peptide with sequence C-KQKLRNNDLKKQN, from the internal region of the protein sequence according to NP_082111.1. Epitope: Internal region

23956208

NP_082111.1

NM_027835.2

110,842 Da

Hepatitis B Pathway (694729); Herpes Simplex Infection Pathway (377874); Herpes Simplex Infection Pathway (377865); Influenza A Pathway (217174); Influenza A Pathway (217150); Measles Pathway (213308); Measles Pathway (213277); RIG-I-like Receptor Signaling Pathway (117302); RIG-I-like Receptor Signaling Pathway (116128)

IFIH1: Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O- methylation at their 5' cap and long-dsRNA ( 1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines. Monomer in the absence of ligands and homodimerizes in the presence of dsRNA ligands. Can assemble into helical or linear polymeric filaments on long dsRNA. Interacts with MAVS/IPS1. Interacts with V protein of Simian virus 5, Human parainfluenza virus 2, Mumps virus, Sendai virus and Hendra virus. Binding to paramyxoviruses V proteins prevents IFN-beta induction, and the further establishment of an antiviral state. Interacts with PCBP2. Interacts with NLRC5. Interacts with PIAS2-beta. Interacts with DDX60. By interferon (IFN) and TNF. Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung. Belongs to the helicase family. RLR subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.6.4.13; Apoptosis; Helicase. Cellular Component: cytoplasm; nucleus. Molecular Function: ATP binding; DNA binding; double-stranded RNA binding; helicase activity; hydrolase activity; hydrolase activity, acting on acid anhydrides; metal ion binding; nucleotide binding; protein binding; ribonucleoprotein binding; RNA binding; single-stranded RNA binding; zinc ion binding. Biological Process: defense response to virus; immune system process; innate immune response; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; protein sumoylation; response to virus

0.1 mg

225 USD