catalog number :
MBS420598
products full name :
Goat anti-Perilipin (C Terminus) Antibody
products short name :
Perilipin
products name syn :
Plin; perilipin; PERI; lipid droplet-associated protein; Goat Anti-Perilipin (internal); Plin; perilipin; PERI; lipid droplet-associated protein
other names :
perilipin; Perilipin-1; perilipin-1; lipid droplet-associated protein; perilipin 1; Lipid droplet-associated protein
products gene name :
PLIN
other gene names :
PLIN1; PLIN1; PERI; PLIN; FPLD4; PERI; PLIN
uniprot entry name :
PLIN1_HUMAN
reactivity :
Human, Rat, Dog
sequence :
EPILGRAQYSQLRKK
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
app notes :
Western Blot: Approx 55-60kDa band observed in Human Adipose lysates (calculated MW of 56.0kDa according to NP_002657.2). Recommended concentration: 0.01-0.03 ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Immunofluorescence: Small lipid droplets preferentially stained in the periphery of differentiated 3T3-L1 adipocytes. Recommended concentration 1ug/ml.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-EPILGRAQYSQLRKK, from the C Terminus of the protein sequence according to NP_002657.2.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425633
ncbi acc num :
NP_002657.2
ncbi gb acc num :
NM_002666.3
ncbi mol weight :
55,990 Da
ncbi pathways :
Adipogenesis Pathway 198832!!Developmental Biology Pathway 477129!!Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway 106121!!Lipid Digestion, Mobilization, And Transport Pathway 106111!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976!!PPAR Signaling Pathway 83042!!PPAR Signaling Pathway 450!!Transcriptional Regulation Of White Adipocyte Differentiation Pathway 205243
ncbi summary :
The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009]