Goat anti-GPX4 (Isoform a and c) Antibody | MyBioSource MBS420590 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-GPX4 (Isoform a and c) Antibody

catalog :

MBS420590

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420590

products type :

Antibody

products full name :

Goat anti-GPX4 (Isoform a and c) Antibody

products short name :

GPX4

products name syn :

GPX4; glutathione peroxidase 4 (phospholipid hydroperoxidase); HGNC: 4556; PHGPx; snGPx; glutathione peroxidase 4; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase; GPX4 antibody; glutathione peroxidase 4 (phospholipid hydroperoxidase) antibody; HGNC: 4556 antibody; PHGPx antibody; snGPx antibody; glutathione peroxidase 4 antibody; phospholipid hydroperoxidase antibody; sperm nucleus glutathione peroxidase antibody; GPX4 (Isoform a and c)

other names :

phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform A; Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; glutathione peroxidase 4; Glutathione peroxidase 4; GPx-4; GSHPx-4

products gene name :

GPX4

other gene names :

GPX4; GPX4; MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx; PHGPx; GPx-4; GSHPx-4

uniprot entry name :

GPX4_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

sequence length :

197

sequence :

EEPLVIEKDLPHY

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3ug/ml. Western Blot: Approx 19kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2). Recommended concentration: 0.1-0.3ug/ml.

other info1 :

Immunogen: Peptide with sequence C-EEPLVIEKDLPHY, from the C Terminus of the protein sequence according to NP_002076.2; NP_001034937.1. Epitope: C Terminus

other info2 :

Note: This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).

ncbi gi num :

75709200

ncbi acc num :

NP_002076.2

ncbi gb acc num :

NM_002085.4

ncbi mol weight :

19,525 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (1270087); Folate Metabolism Pathway (198833); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Selenium Metabolism And Selenoproteins Pathway (198846); Selenium Pathway (198825); Synthesis Of 12-eicosatetraenoic Acid Derivatives Pathway (1270092)

ncbi summary :

This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

uniprot summary :

GPX4: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative initiation. Protein type: Lipid Metabolism - arachidonic acid; Oxidoreductase; Mitochondrial; EC 1.11.1.12; Nuclear envelope; Other Amino Acids Metabolism - glutathione. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: cytosol; mitochondrion; nucleus. Molecular Function: glutathione peroxidase activity; phospholipid-hydroperoxide glutathione peroxidase activity. Biological Process: lipoxygenase pathway; multicellular organismal development; phospholipid metabolic process; response to oxidative stress

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage