catalog number :
MBS420585
products full name :
Goat anti-Semaphorin 3E (internal) Antibody
products short name :
Semaphorin 3E
products name syn :
SEMA3E; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; (semaphorin) 3E; KIAA0331; M-SEMAH; M-SemaK; M-sema H; SEMAH; coll-5; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; 3E; semaphorin 3E; Goat Anti-SEMA3E; SEMA3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; KIAA0331; M-SEMAH; M-SemaK; M-sema H; SEMAH; coll-5; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E; semaphorin 3E
other names :
semaphorin-3E isoform 1; Semaphorin-3E; semaphorin-3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
products gene name :
SEMA3E
other gene names :
SEMA3E; SEMA3E; SEMAH; coll-5; M-SEMAH; M-SemaK; KIAA0331; SEMAH
uniprot entry name :
SEM3E_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Pig
sequence :
NGGRYGTTKDYPDD
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
app notes :
Immunohistochemistry: In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland. Recommended concentration, 4-6ug/ml. Western Blot: Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0.03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. Peptide ELISA: antibody detection limit dilution 1:128000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-NGGRYGTTKDYPDD, from the internal region of the protein sequence according to NP_036563.1; NP_001171600.1. Corresponding Immunizing Peptide: Catalog #MBS426205
other info2 :
Important Note: This antibody is expected to recognise both reported isoforms (NP_036563.1; NP_001171600.1).
ncbi acc num :
NP_036563.1
ncbi gb acc num :
NM_012431.2
ncbi mol weight :
82,424 Da
ncbi pathways :
Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!Axon Guidance Pathway 105688!!Developmental Biology Pathway 477129!!Other Semaphorin Interactions Pathway 119526!!Semaphorin Interactions Pathway 119519
ncbi summary :
Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]