Goat anti-GOT1 (aa 157-167) Antibody | MyBioSource MBS420584 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-GOT1 (aa 157-167) Antibody

catalog :

MBS420584

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420584

products type :

Antibody

products full name :

Goat anti-GOT1 (aa 157-167) Antibody

products short name :

GOT1

products name syn :

Goat Anti-GOT1 (aa 22-35) Antibody; GOT1; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); GIG18; aspartate aminotransferase 1; growth-inhibiting protein 18; GOT1 antibody; glutamic-oxaloacetic transaminase 1; soluble (aspartate aminotransferase 1) antibody; GIG18 antibody; aspartate aminotransferase 1 antibody; growth-inhibiting protein 18 antibody; GOT1 (aa 157-167)

other names :

aspartate aminotransferase, cytoplasmic; Aspartate aminotransferase, cytoplasmic; aspartate aminotransferase, cytoplasmic; glutamic-oxaloacetic transaminase 1; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A

products gene name :

GOT1

other gene names :

GOT1; GOT1; AST1; cCAT; GIG18; cAspAT; ASTQTL1; cAspAT; cCAT

uniprot entry name :

AATC_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Rat

sequence length :

413

sequence :

RSYRYWDAEKR

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded Human Heart shows strong staining of the capillary network between the myocardial fibres in transverse secion. Recommended concentration, 2-3ug/ml. Western Blot: Approx 38-40kDa band observed in Human Brain (Cerebellum), Liver and Skeletal Muscle lysates (calculated MW of 46.2kDa according to NP_002070.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Berk et al, Proc Natl Acad Sci U S A.1990 May;87(9): 3484-8; PMID: 2185471). Recommended concentration: 0. 01-1ug/ml.

other info1 :

Immunogen: Peptide with sequence C-RSYRYWDAEKR, from the internal region of the protein sequence according to NP_002070.1. Epitope: Internal region

ncbi gi num :

4504067

ncbi acc num :

NP_002070.1

ncbi gb acc num :

NM_002079.2

ncbi mol weight :

44,147 Da

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (1270159); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Arginine Biosynthesis Pathway (82943); Arginine Biosynthesis Pathway (306)

ncbi summary :

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

uniprot summary :

GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Protein type: Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - cysteine and methionine; EC 2.6.1.1; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.3; Transferase; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis. Chromosomal Location of Human Ortholog: 10q24.1-q25.1. Cellular Component: cytoplasm; cytosol; lysosome; mitochondrion; nerve terminal; nucleus. Molecular Function: aspartate transaminase activity; carboxylic acid binding; cysteine transaminase activity; identical protein binding; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding. Biological Process: 2-oxoglutarate metabolic process; amino acid biosynthetic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; cellular response to insulin stimulus; fatty acid homeostasis; gluconeogenesis; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glutamate metabolic process; glycerol biosynthetic process; Notch signaling pathway; oxaloacetate metabolic process; response to glucocorticoid stimulus. Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage