Goat anti-Dynactin Antibody | MyBioSource MBS420581 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-Dynactin Antibody

catalog :

MBS420581

quantity :

0.1 mg

price :

290 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420581

products type :

Antibody

products full name :

Goat anti-Dynactin Antibody

products short name :

Dynactin

products name syn :

DCTN1; dynactin 1 (p150; glued homolog; Drosophila); P135; DP-150; DAP-150; p150-glued; p150; Glued (Drosophila) homolog; 150 kDa dynein-associated polypeptide; dynactin 1 (p150; Glued (Drosophila) homolog); HMN7B; OTTHUMP00000202491; dynactin 1; p150; Glued homolog; DCTN1; dynactin 1 (p150, glued homolog, Drosophila); P135; DP-150; DAP-150; p150-glued; p150, Glued (Drosophila) homolog; 150 kDa dynein-associated polypeptide; dynactin 1 (p150, Glued (Drosophila) homolog); HMN7B; OTTHUMP00000202491; dynactin 1; p150, Glued homolog

other names :

dynactin subunit 1 isoform 1; Dynactin subunit 1; dynactin subunit 1; 150 kDa dynein-associated polypeptide; dynactin 1 (p150, glued homolog, Drosophila); dynactin 1; 150 kDa dynein-associated polypeptide; DAP-150; DP-150; p135; p150-glued

products gene name :

DCTN1

other gene names :

DCTN1; DCTN1; P135; DP-150; DAP-150; DP-150

uniprot entry name :

DCTN1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Human, Mouse, Rat

sequence length :

1278

sequence :

QEQLHQLHSRLIS

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

app notes :

Immunohistochemistry: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4ug/ml. An anonymous customer found positive results in IHC on Human HeLa cells. Western Blot: Approx 150kDa band observed in cell lline A549 and in Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2ug/ml. An anonymous customer found positive results in WB on Mouse embryonic fibroblasts (MEF cells). An anonymous customer found positive results in WB on Human HeLa cells. Peptide ELISA: antibody detection limit dilution 1:128000. Immunocytochemsitry: An anonymous customer found positive results in ICC on Mouse embryonic fibroblasts (MEF cells).

other info1 :

Epitope: C Terminus. Immunogen: Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1. Corresponding Immunizing Peptide: Catalog #MBS425873

other info2 :

Important Note: This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).

products references :

Bupha-Intr T, Haizlip KM, Janssen PM.; Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro.; Am J Physiol Heart Circ Physiol. 2009 Mar; 296(3):H806-14.; Pubmed 19136602

ncbi gi num :

13259510

ncbi acc num :

NP_004073.2

ncbi gb acc num :

NM_004082.4

ncbi mol weight :

140,887 Da

ncbi pathways :

Adaptive Immune System Pathway 366160!!Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Centrosome Maturation Pathway 105807!!G2/M Transition Pathway 105801!!Huntington's Disease Pathway 83100!!Huntington's Disease Pathway 512!!IRE1alpha Activates Chaperones Pathway 105906!!Immune System Pathway 106386!!Loss Of Nlp From Mitotic Centrosomes Pathway 105811

ncbi summary :

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]

size :

0.1 mg

price :

290 USD

more info or order :

MyBioSource product webpage