Goat anti-NONO/p54NRB Antibody | MyBioSource MBS420569 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-NONO/p54NRB Antibody

catalog :

MBS420569

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420569

products type :

Antibody

products full name :

Goat anti-NONO/p54NRB Antibody

products short name :

NONO/p54NRB

products name syn :

NONO; P54NRB; non-POU domain containing, octamer-binding; HGNC: 7871; NMT55; NRB54; P54; Nuclear RNA-binding protein, 54-kD; non-POU-domain-containing, octamer-binding; non-Pou domain-containing octamer (ATGCAAAT) binding protein; NONO antibody; P54NRB antibody; non-POU domain containing; octamer-binding antibody; HGNC: 7871 antibody; NMT55 antibody; NRB54 antibody; P54 antibody; Nuclear RNA-binding protein; 54-kD antibody; non-POU-domain-containing; octamer-binding antibody; non-Pou domain-containing octamer (ATGCAAAT) binding protein antibody; NONO / p54NRB

other names :

non-POU domain-containing octamer-binding protein isoform 1; Non-POU domain-containing octamer-binding protein; non-POU domain-containing octamer-binding protein; non-POU domain containing, octamer-binding; 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; NMT55; p54(nrb); p54nrb

products gene name :

NONO

products gene name syn :

p54NRB

other gene names :

NONO; NONO; P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114; NRB54; NonO protein; p54nrb

uniprot entry name :

NONO_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog

sequence length :

471

sequence :

NRAAPGAEFAPNK

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining in select splenocytes. Recommended concentration, 3-6ug/ml. Western Blot: Approx 60kDa band observed in Mouse Brain lysates (calculated MW of 54.2kDa according to Human NP_031389.3 and 54.5kDa according to Mouse NP_075633.2). In transfected HEK293 transiently expressing Human NONO a band of approx.55kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 50kDa protein is observed in both. Recommended concentration: 0.1-0.3ug/ml.

other info1 :

Immunogen: Peptide with sequence C-NRAAPGAEFAPNK, from the C Terminus of the protein sequence according to NP_031389.3. Epitope: C Terminus

ncbi gi num :

34932414

ncbi acc num :

NP_031389.3

ncbi gb acc num :

NM_007363.4

ncbi mol weight :

43,866 Da

ncbi pathways :

Circadian Rhythm Pathway (137978); MRNA Processing Pathway (198843)

ncbi summary :

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]

uniprot summary :

NONO: DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Together with PSPC1, required for the formation of nuclear paraspeckles. A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3. Protein type: Nucleolus; RNA-binding; Nuclear receptor co-regulator; RNA splicing. Chromosomal Location of Human Ortholog: Xq13.1. Cellular Component: membrane; nuclear matrix; nuclear speck; nucleolus; nucleoplasm; nucleus; paraspeckles. Molecular Function: chromatin binding; identical protein binding; nucleotide binding; protein binding. Biological Process: circadian rhythm; DNA recombination; DNA repair; mRNA processing; negative regulation of transcription, DNA-dependent; nuclear mRNA splicing, via spliceosome; regulation of circadian rhythm; regulation of transcription, DNA-dependent; RNA splicing; transcription, DNA-dependent

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage