catalog number :
MBS420558
products full name :
Goat anti-FENS1/WDFY1 Antibody
products short name :
FENS1/WDFY1
products name syn :
WDFY1; WDF1; FENS-1; KIAA1435; WD repeat and FYVE domain containing 1; WD40 and FYVE domain containing 1; phosphoinositide-binding protein SR1; ZFYVE17; WDFY1 antibody; WDF1 antibody; FENS-1 antibody; KIAA1435 antibody; WD repeat and FYVE domain containing 1 antibody; WD40 and FYVE domain containing 1 antibody; phosphoinositide-binding protein SR1 antibody; ZFYVE17 antibody; FENS1 / WDFY1
other names :
WD repeat and FYVE domain-containing protein 1; WD repeat and FYVE domain-containing protein 1; WD repeat and FYVE domain-containing protein 1; WD repeat and FYVE domain containing 1; FENS-1; Phosphoinositide-binding protein 1; WD40- and FYVE domain-containing protein 1; Zinc finger FYVE domain-containing protein 17
products gene name :
WDFY1
products gene name syn :
FENS1
other gene names :
WDFY1; WDFY1; WDF1; FENS-1; ZFYVE17; KIAA1435; WDF1; ZFYVE17
uniprot entry name :
WDFY1_HUMAN
reactivity :
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunohistochemistry (IHC)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatoicytes. Recommended concentration, 4-6ug/ml. Western Blot: Preliminary experiments gave no signal but low background in Human Liver and HepG2 lysates at up to 1ug/ml.
other info1 :
Immunogen: Peptide with sequence VVGCSLATGFSPH, from the C Terminus of the protein sequence according to NP_065881.1. Epitope: C Terminus
other info2 :
Note: This antibody is expected to recognise both reported isoforms (as represented by NP_065881 and NP_848127)
ncbi acc num :
NP_065881.1
ncbi gb acc num :
NM_020830.4
ncbi mol weight :
46,324 Da
ncbi summary :
The protein encoded by this gene is a phosphatidylinositol 3-phosphate binding protein, which contains a FYVE zinc finger domain and multiple WD-40 repeat domains. When exogenously expressed, it localizes to early endosomes. Mutagenesis analysis demonstrates that this endosomal localization is mediated by the FYVE domain. [provided by RefSeq, Jan 2015]
uniprot summary :
WDFY1: Binds PtdIns3P in vitro with high specificity over other phosphoinositides. Chromosomal Location of Human Ortholog: 2q36.1. Cellular Component: cytosol; early endosome; nucleus. Molecular Function: phosphatidylinositol binding; protein binding; zinc ion binding. Biological Process: positive regulation of toll-like receptor 3 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway