antibody

Goat anti-LRP6 Antibody

MBS420538

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-LRP6 Antibody

LRP6

LRP6; low density lipoprotein receptor-related protein 6; low density lipoprotein-related protein 6; ADCAD2; FLJ90062; FLJ90421; LRP6 antibody; low density lipoprotein receptor-related protein 6 antibody; low density lipoprotein-related protein 6 antibody

low-density lipoprotein receptor-related protein 6; Low-density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LDL receptor related protein 6

LRP6

LRP6; LRP6; ADCAD2; STHAG7; LRP-6

LRP6_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog

1613

CMTSVATAKGYTSDL

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Kidney shows luminar membrane staining in DCT. Recommended concentration, 4-6ug/ml. Western Blot: Preliminary experiments gave bands at approx 50kDa and 35kDa in Human Adipose lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 180kDa according to NP_002327.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Immunogen: Peptide with sequence CMTSVATAKGYTSDL, from the internal region of the protein sequence according to NP_002327.2. Epitope: Internal region

Note: This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein.

148727288

NP_002327.2

NM_002336.2

180,429 Da

Canonical Wnt Signaling Pathway (138032); Disassembly Of The Destruction Complex And Recruitment Of AXIN To The Membrane Pathway (1269601); Disease Pathway (1268854); Diseases Of Signal Transduction Pathway (1268855); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); Negative Regulation Of TCF-dependent Signaling By WNT Ligand Antagonists Pathway (1269608); Presenilin Action In Notch And Wnt Signaling Pathway (137914); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1268924); Regulation Of FZD By Ubiquitination Pathway (1269607); Signal Transduction Pathway (1269379)

This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.[provided by RefSeq, Dec 2009]

LRP6: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation. Homodimer; disulfide-linked. Forms phosphorylated oligomer aggregates on Wnt-signaling. Forms a WNT-signaling complex formed of a WNT protein, a FZD protein and LRP5 or LRP6. Interacts (via the extracellular domain) with WNT1; the interaction is enhanced by prior formation of the Wnt/Fzd complex. Interacts (via the beta-propeller regions 3 and 4) with WNT3A. Interacts (via the beta-propeller regions 1 and 2) with WNT9B. Interacts with FZD5; the interaction forms a coreceptor complex for Wnt signaling and is inhibited by DKK1 and C1orf187. Interacts (via beta propeller region) with DKK1; the interaction inhibits FZD5/LRP6 complex formation. Interacts with DKK2. Interacts with C1orf187/DRAXIN; the interaction inhibits Wnt signaling. Interacts (via the phosphorylated PPPSP motifs) with AXIN1; the interaction recruits the AXIN1/GSK3B complex to cell surface LRP6 signalsomes. Interacts with GRB10; the interaction prevents AXIN1 binding, thus negatively regulating the Wnt signaling pathway. Interacts (via the extracellular domain) with RSPO1; the interaction activates Wnt/beta-catenin signaling. Interacts (via the extracellular domain) with RSPO3 (via the cysteine rich domain); the interaction activates Wnt/beta-catenin signaling. Interacts (via the beta- propeller regions 1 and 2) with SOST; the interaction competes with DKK1 for binding for inhibiting beta-catenin signaling. Interacts with MESD; the interaction prevents the formation of LRP6 aggregates and targets LRP6 to the plasma membrane. Interacts (via the cytoplasmic domain) with CSNKIE; the interaction phosphorylates LRP6, binds AXIN1 and inhibits AXIN1/GSK3B-mediated phosphorylation of beta-catenin. Interacts with MACF1. Decreased levels on WNT3A stimulation. Widely co-expressed with LRP5 during embryogenesis and in adult tissues. Belongs to the LDLR family. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.2. Cellular Component: caveola; cell soma; cell surface; cytoplasmic vesicle; cytosol; early endosome; early endosome membrane; endoplasmic reticulum; extracellular region; Golgi apparatus; integral to membrane; plasma membrane; receptor complex; synapse. Molecular Function: apolipoprotein binding; frizzled binding; identical protein binding; kinase inhibitor activity; low-density lipoprotein receptor activity; protein binding; protein homodimerization activity; receptor binding; toxin transporter activity; Wnt receptor activity; Wnt-protein binding. Biological Process: cell-cell adhesion; cerebellum morphogenesis; cerebral cortex development; convergent extension; elevation of cytosolic calcium ion concentration; embryonic pattern specification; embryonic retina morphogenesis in camera-type eye; external genitalia morphogenesis; midbrain-hindbrain boundary development; negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase activity; neural crest cell differentiation; neural crest formation; neural tube closure; odontogenesis of dentine-containing teeth; palate development; positive regulation of cell cycle; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to peptide hormone stimulus; synaptic transmission; thalamus development; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin. Disease: Coronary Artery Disease, Autosomal Dominant 2

0.1 mg

300 USD