Goat anti-Arrestin beta 2 Antibody | MyBioSource MBS420529 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-Arrestin beta 2 Antibody

catalog :

MBS420529

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420529

products type :

Antibody

products full name :

Goat anti-Arrestin beta 2 Antibody

products short name :

Arrestin beta 2

products name syn :

ARRB2; arrestin, beta 2; HGNC: 712; ARB2; ARR2; DKFZp686L0365; arrestin beta 2; beta-arrestin 2; BARR2; arrestin 3; arrestin; beta 2 antibody; ARRB2 antibody; HGNC: 712 antibody; ARB2 antibody; ARR2 antibody; DKFZp686L0365 antibody; arrestin beta 2 antibody; beta-arrestin 2 antibody; BARR2 antibody; Arrestin beta 2; Arrestin beta 2

other names :

beta-arrestin-2 isoform 1; Beta-arrestin-2; beta-arrestin-2; arrestin beta 2; Arrestin beta-2

products gene name :

ARRB2

other gene names :

ARRB2; ARRB2; ARB2; ARR2; BARR2; ARB2; ARR2

uniprot entry name :

ARRB2_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat

sequence length :

409

sequence :

HDHIPLPRPQS

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Cerebellum shows mainly nuclear staining of purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration, 4-6ug/ml. Western Blot: Approx 40kDa band observed in Human Brain lysates (calculated MW of 46.1kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1ug/ml. An anonymous customer found positive results in WB on Rat aortic smooth muscle. An anonymous customer found positive results in WB on Mouse smooth muscle. An anonymous customer found positive results in WB on Mouse aortic smooth muscle. An anonymous customer found positive results in WB on Human colon adenocarcinoma cells (SW480). Immunoprecipitation: This antibody has been successfully used in IP from rat leukocytes as described in the following paper: Zakrzewicz et al, Immunobiology.2011 Jul;216(7): 854-61; PMID: 21193245.

other info1 :

Immunogen: Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1; NP_945355.1. Epitope: Internal region

other info2 :

Note: This antibody is expected to recognise both reported isoforms NP_004304.1 and NP_945355.1. No crossreactivity is expected with Arrestin beta 1.

ncbi gi num :

4757780

ncbi acc num :

NP_004304.1

ncbi gb acc num :

NM_004313.3

ncbi mol weight :

44,392 Da

ncbi pathways :

ALK1 Signaling Events Pathway (137968); ARMS-mediated Activation Pathway (1269471); Activated NOTCH1 Transmits Signal To The Nucleus Pathway (1269536); Activation Of SMO Pathway (1269642); Arf6 Signaling Events Pathway (138034); Atypical NF-kappaB Pathway (137946); Axon Guidance Pathway (1270303); Beta-catenin Independent WNT Signaling Pathway (1269610); CXCR4-mediated Signaling Events Pathway (137910); Calcium Regulation In The Cardiac Cell Pathway (198906)

ncbi summary :

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

uniprot summary :

ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 17p13. Cellular Component: basolateral plasma membrane; coated pit; cytoplasm; cytoplasmic vesicle; cytosol; dendritic spine; endocytic vesicle; nucleus; plasma membrane; postsynaptic density; postsynaptic membrane. Molecular Function: alpha-1A adrenergic receptor binding; alpha-1B adrenergic receptor binding; angiotensin receptor binding; D1 dopamine receptor binding; enzyme binding; follicle stimulating hormone receptor binding; G-protein-coupled receptor binding; mitogen-activated protein kinase binding; platelet activating factor receptor binding; protein binding; protein complex binding; protein complex scaffold; protein domain specific binding; protein kinase B binding; receptor binding; type 1 angiotensin receptor binding; type 2A serotonin receptor binding; ubiquitin protein ligase binding. Biological Process: adult walking behavior; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; brain development; detection of temperature stimulus involved in sensory perception of pain; dopamine receptor signaling pathway; follicle-stimulating hormone signaling pathway; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; negative regulation of caspase activity; negative regulation of interleukin-1 beta production; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of natural killer cell mediated cytotoxicity; negative regulation of protein kinase B signaling cascade; negative regulation of protein ubiquitination; negative regulation of toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; platelet activation; positive regulation of calcium ion transport; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of protein ubiquitination; positive regulation of receptor internalization; positive regulation of synaptic transmission, dopaminergic; proteasomal ubiquitin-dependent protein catabolic process; protein transport; protein ubiquitination; receptor internalization; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage