Goat anti-VDP p115 Antibody | MyBioSource MBS420500 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-VDP p115 Antibody

catalog :

MBS420500

quantity :

0.1 mg

price :

305 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

(0.3ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

product information

catalog number :

MBS420500

products type :

Antibody

products full name :

Goat anti-VDP p115 Antibody

products short name :

[VDP p115]

products name syn :

[VDP p115, vesicle docking protein, P115, TAP, transcytosis-associated protein, USO1, USO1 homolog, vesicle docking protein (yeast), USO1 homolog, vesicle docking protein, transcytosis associated protein, vesicle docking protein p115]

other names :

[general vesicular transport factor p115; General vesicular transport factor p115; general vesicular transport factor p115; USO1 vesicle transport factor; Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein]

products gene name :

[USO1]

products gene name syn :

[VDP p115]

other gene names :

[USO1; USO1; TAP; VDP; P115; VDP; TAP]

uniprot entry name :

USO1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human

sequence length :

962

sequence :

DESEDPGKDLDH

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx 110kDa band observed in lysates of cell line K562 (calculated MW of 108kDa according to NP_003706.1). Recommended concentration: 0.3-1ug/ml.

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: Peptide with sequence C-DESEDPGKDLDH, from the C Terminus of the protein sequence according to NP_003706.1.

ncbi gi num :

4505541

ncbi acc num :

NP_003706.1

ncbi gb acc num :

NM_003715.2

ncbi mol weight :

109,195 Da

ncbi pathways :

Arf1 Pathway (137927); Asparagine N-linked Glycosylation Pathway (1268714); COPI-mediated Anterograde Transport Pathway (1339109); COPII (Coat Protein 2) Mediated Vesicle Transport Pathway (1268727); Cell Cycle Pathway (1269741); Cell Cycle, Mitotic Pathway (1269763); ER To Golgi Anterograde Transport Pathway (1268726); Golgi Cisternae Pericentriolar Stack Reorganization Pathway (1269812); M Phase Pathway (1269810); Membrane Trafficking Pathway (1269877)

ncbi summary :

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

uniprot summary :

TAP: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Belongs to the VDP/USO1/EDE1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Vesicle; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q21.1. Cellular Component: cytosol; endoplasmic reticulum; Golgi apparatus; Golgi membrane; Golgi stack; membrane; nucleolus; perinuclear region of cytoplasm; transport vesicle. Molecular Function: protein binding; protein transporter activity. Biological Process: COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; Golgi vesicle docking; intracellular protein transport; transcytosis; vesicle fusion with Golgi apparatus

size1 :

0.1 mg

price1 :

305 USD

more info or order :

MyBioSource product webpage