catalog number :
MBS420481
products full name :
Goat anti-Sorbitol Dehydrogenase Antibody
products short name :
Sorbitol Dehydrogenase
products name syn :
sorbitol dehydrogenase; SORD; SORD1; sorbitol dehydrogenase; SORD; SORD1
other names :
sorbitol dehydrogenase; Sorbitol dehydrogenase; sorbitol dehydrogenase; L-iditol 2-dehydrogenase; epididymis secretory sperm binding protein Li 95n; sorbitol dehydrogenase; L-iditol 2-dehydrogenase
products gene name :
SORD
other gene names :
SORD; SORD; SORD1; HEL-S-95n
uniprot entry name :
DHSO_HUMAN
reactivity :
Human, Mouse, Rat, Dog
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 38kDa band observed in Mouse Kidney lysates (calculated MW of 38.3kDa according to NP_003095.1). Recommended concentration: 0.1-0.3ug/ml. A minor band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. Peptide ELISA: antibody detection limit dilution 1:16000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence KIMLKCDPSDQNP, from the C Terminus of the protein sequence according to NP_003095.2.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS426174
products references :
Cao W, Aghajanian HK, Haig-Ladewig LA, Gerton GL.; Sorbitol can fuel mouse sperm motility and protein tyrosine phosphorylation via sorbitol dehydrogenase.; Biol Reprod. 2009 Jan; 80(1):124-33.; Pubmed 18799757
ncbi acc num :
NP_003095.2
ncbi gb acc num :
NM_003104.5
ncbi mol weight :
10,812 Da
ncbi pathways :
Fructose And Mannose Metabolism Pathway 82930!!Fructose And Mannose Metabolism Pathway 291!!Glucuronate Pathway (uronate Pathway) 997799!!Glucuronate Pathway (uronate Pathway) 468207!!Metabolic Pathways 132956!!Pentose And Glucuronate Interconversions Pathway 82929!!Pentose And Glucuronate Interconversions Pathway 290!!Polyol Pathway 198815!!Sorbitol Degradation I Pathway 142439
ncbi summary :
Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]