Goat anti-PCK1/PEPCKC (internal region) Antibody | MyBioSource MBS420472 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-PCK1/PEPCKC (internal region) Antibody

catalog :

MBS420472

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420472

products type :

Antibody

products full name :

Goat anti-PCK1/PEPCKC (internal region) Antibody

products short name :

PCK1/PEPCKC

products name syn :

Goat Anti-PCK1 / PEPCKC (internal) Antibody; PCK1; PEPCKC; phosphoenolpyruvate carboxykinase 1 (soluble); MGC22652; PEPCK1; PEP carboxykinase; cytosolic phosphoenolpyruvate carboxykinase 1; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; PCK1 antibody; PEPCKC antibody; phosphoenolpyruvate carboxykinase 1 (soluble) antibody; MGC22652 antibody; PEPCK1 antibody; PEP carboxykinase antibody; cytosolic phosphoenolpyruvate carboxykinase 1 antibody; phosphoenolpyruvate carboxylase antibody; phosphopyruvate carboxylase antibody; PCK1 / PEPCKC (internal region)

other names :

cytosolic phosphoenolpyruvate carboxykinase 1; Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxykinase 1

products gene name :

PCK1

products gene name syn :

PEPCKC

other gene names :

PCK1; PCK1; PEPCK1; PEPCKC; PEPCK-C; PEPCK-C

uniprot entry name :

PCKGC_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human

sequence length :

622

sequence :

EKEVEDIEKYLEDQ

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 70kDa band observed in Human Kidney lysates (calculated MW of 69.2kDa according to NP_002582.2). Recommended concentration: 0.5-1.5ug/ml.

other info1 :

Immunogen: Peptide with sequence C-EKEVEDIEKYLEDQ, from the internal region (near the C Terminus) of the protein sequence according to NP_002582.2. Epitope: Internal region (near the C Terminus)

ncbi gi num :

32483401

ncbi acc num :

NP_002582.2

ncbi gb acc num :

NM_002591.2

ncbi mol weight :

34,156 Da

ncbi pathways :

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Abacavir Metabolism Pathway (1270228); Abacavir Transport And Metabolism Pathway (1270226); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Developmental Biology Pathway (1270302)

ncbi summary :

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

PCK1: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; Carbohydrate Metabolism - pyruvate; Kinase, other; Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.1.1.32. Chromosomal Location of Human Ortholog: 20q13.31. Cellular Component: cytoplasm; cytosol. Molecular Function: carboxylic acid binding; GDP binding; GTP binding; magnesium ion binding; manganese ion binding; phosphoenolpyruvate carboxykinase (GTP) activity. Biological Process: cellular response to potassium ion starvation; gluconeogenesis; glucose homeostasis; glucose metabolic process; glycerol biosynthetic process from pyruvate; internal protein amino acid acetylation; oxaloacetate metabolic process; response to activity; response to insulin stimulus. Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Cytosolic

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage