catalog number :
MBS420469
products full name :
Goat anti-Cytochrome b reductase 1 Antibody
products short name :
Cytochrome b reductase 1
products name syn :
CYBRD1; DCYTB; FLJ23462; cytochrome b reductase 1; duodenal cytochrome b; FRRS3; ferric-chelate reductase 3; CYBRD1; DCYTB; FLJ23462; cytochrome b reductase 1; duodenal cytochrome b; FRRS3; ferric-chelate reductase 3
other names :
cytochrome b reductase 1 isoform 1; Cytochrome b reductase 1; cytochrome b reductase 1; duodenal cytochrome b; ferric-chelate reductase 3; cytochrome b561 family, member A2; cytochrome b reductase 1; Duodenal cytochrome b; Ferric-chelate reductase 3
products gene name :
CYBRD1
other gene names :
CYBRD1; CYBRD1; DCYTB; FRRS3; CYB561A2; DCYTB; FRRS3
uniprot entry name :
CYBR1_HUMAN
reactivity :
Human, Dog, Pig, Cow, Mouse
sequence :
CRNLALDEAGQRSTM
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
app notes :
Immunohistochemistry: In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration, 2-5ug/ml. An anonymous customer found positive results in IHC on Mouse duodenum. Western Blot: Experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 31.6kDa according to NP_079119. The detected bands were all successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Peptide ELISA: antibody detection limit dilution 1:16000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence CRNLALDEAGQRSTM, from the C Terminus of the protein sequence according to NP_079119.3. Corresponding Immunizing Peptide: Catalog #MBS425013
other info2 :
Important Note: This antibody is expected to recognise isoform 1 (NP_079119.3) only.
ncbi acc num :
NP_079119.3
ncbi gb acc num :
NM_024843.3
ncbi mol weight :
25,087 Da
ncbi pathways :
Iron Uptake And Transport Pathway 187191!!Mineral Absorption Pathway 212237!!Mineral Absorption Pathway 212220!!Transmembrane Transport Of Small Molecules Pathway 106572
ncbi summary :
This gene is a member of the cytochrome b(561) family that encodes an iron-regulated protein. It highly expressed in the duodenal brush border membrane. It has ferric reductase activity and is believed to play a physiological role in dietary iron absorption. [provided by RefSeq, Jul 2008]