catalog number :
MBS420442
products full name :
Goat anti-AGRP/Agouti-related protein Antibody
products short name :
AGRP/Agouti-related protein
products name syn :
AGRP; ART; AGRT; ASIP2; agouti related protein homolog (mouse); agouti (mouse) related protein; Agouti-related transcript, mouse, homolog of; MGC118963; agouti related protein homolog; AGRP antibody; ART antibody; AGRT antibody; ASIP2 antibody; agouti related protein homolog (mouse) antibody; agouti (mouse) related protein antibody; Agouti-related transcript; mouse; homolog of antibody; MGC118963 antibody; agouti related protein homolog antibody; Agouti-related protein; AGRP / Agouti-related protein
other names :
agouti-related protein; Agouti-related protein; agouti-related protein; agouti related neuropeptide
products gene name :
AGRP
other gene names :
AGRP; AGRP; ART; AGRT; ASIP2; AGRT; ART
uniprot entry name :
AGRP_HUMAN
reactivity :
Tested: Human; Expected from sequence similarity: Human, Dog, Pig
sequence :
CRKLGTAMNPCSRT
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunohistochemistry (IHC)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: Frozen section of Human Hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons. Recommended concentration, 0.05-0.1ug/ml. Western Blot: Preliminary experiments gave bands at approx 45kDa and 24kDa in Human Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.4kDa according to NP_001129.1and NP_015531.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
other info1 :
Immunogen: Peptide with sequence CRKLGTAMNPCSRT, from the C Terminus of the protein sequence according to NP_001129.1; NP_015531.1. Epitope: C Terminus
other info2 :
Note: This antibody is expected to recognise both reported isoforms (as represented by NP_001129; NP_015531)
ncbi acc num :
NP_001129.1
ncbi gb acc num :
NM_001138.1
ncbi mol weight :
14,440 Da
ncbi pathways :
Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Syndecan-3-mediated Signaling Events Pathway (137952)
ncbi summary :
This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]