catalog number :
MBS420419
products full name :
Goat anti-P4HA1 Antibody
products short name :
P4HA1
products name syn :
P4HA1; procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase); alpha polypeptide I; 4-PH alpha-1; P4HA; prolyl 4-hydroxylase; alpha I subunit; P4HA1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; 4-PH alpha-1; P4HA; prolyl 4-hydroxylase, alpha I subunit
other names :
prolyl 4-hydroxylase subunit alpha-1 isoform 1; Prolyl 4-hydroxylase subunit alpha-1; prolyl 4-hydroxylase subunit alpha-1; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; prolyl 4-hydroxylase, alpha polypeptide I; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1
products gene name :
P4HA1
other gene names :
P4HA1; P4HA1; P4HA; P4HA; 4-PH alpha-1
uniprot entry name :
P4HA1_HUMAN
reactivity :
Human, Mouse, Rat, Dog
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 60kDa band observed in lysates of cell lines HEK293, A431 and NIH3T3 (calculated MW of 61.0kDa according to NP_000908.2 and NP_001017962.1). Recommended concentration: 0.1-0.1ug/ml.
An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-DFARKDEPDAFKE, from the internal region of the protein sequence according to NP_000908.2; NP_001017962.1. Corresponding Immunizing Peptide: Catalog #MBS425404
other info2 :
Important Note: This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).
ncbi acc num :
NP_000908.2
ncbi gb acc num :
NM_000917.3
ncbi mol weight :
58,958 Da
ncbi pathways :
Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!Metabolic Pathways 132956
ncbi summary :
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]