antibody

Goat anti-Kalirin (isoform 2) Antibody

MBS420418

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-Kalirin (isoform 2) Antibody

Kalirin

Goat Anti-KALRN Antibody; KALRN; kalirin, RhoGEF kinase; FLJ16443; HAPIP; TRAD; duo; huntingtin-associated protein interacting protein (duo); serine/threonine kinase with Dbl- and pleckstrin homology domains; hCG_2022551; DUET; DUO; FLJ12332; FLJ18196; FLJ18623; KALRN antibody; kalirin; RhoGEF kinase antibody; FLJ16443 antibody; HAPIP antibody; TRAD antibody; duo antibody; huntingtin-associated protein interacting protein (duo) antibody; serine/threonine kinase with Dbl- and pleckstrin homology domains antibody; hCG_2022551 antibody; DUET antibody; DUO antibody; FLJ12332 antibody; FLJ18196 antibody; FLJ18623 antibody; Kalirin; Kalirin (isoform 2)

kalirin isoform 2; Kalirin; kalirin; kalirin, RhoGEF kinase; Huntingtin-associated protein-interacting protein; Protein Duo; Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain

KALRN

KALRN; KALRN; DUO; CHD5; DUET; TRAD; CHDS5; HAPIP; ARHGEF24; DUET; DUO; HAPIP; TRAD

KALRN_HUMAN

Polyclonal

Goat

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat

1663

RWHLGPGDPFSTYV

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: An anonymous customer found positive results in IHC on Mouse brain tissue (from previous batch, different goat). Western Blot: Approx 200kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates (calculated MW of 192kDa according to NP_003938.1). Recommended concentration: 0.1-0.3ug/ml.

Immunogen: Peptide with sequence C-RWHLGPGDPFSTYV, from the C Terminus of the protein sequence according to NP_003938.1. Epitope: C Terminus

Note: This antibody is expected to recognize isoform 2 (NP_003938.1).

4504335

NP_003938.1

NM_003947.5

141,164 Da

Arf6 Downstream Pathway (138038); Axon Guidance Pathway (1270303); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (1269447); Developmental Biology Pathway (1270302); EPH-Ephrin Signaling Pathway (1270330); EPHB Forward Signaling Pathway (138047); EPHB-mediated Forward Signaling Pathway (1270332); G Alpha (12/13) Signalling Events Pathway (1269579); G Alpha (q) Signalling Events Pathway (1269578); GPCR Downstream Signaling Pathway (1269574)

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with the huntingtin-associated protein 1, which is a huntingtin binding protein that may function in vesicle trafficking. [provided by RefSeq, Apr 2016]

kalirin: a bifunctional enzyme with protein kinase activity and guanine-exchange factor (GEF) activity. A protein kinase of the CAMK group. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity. Genetic variants are associated with susceptibility to coronary heart disease type 5 (CHDS5), a multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Interacts with the C-terminal of peptidylglycine alpha-amidating monooxygenase (PAM) and with the huntingtin-associated protein 1 (HAP1) Interacts with FASLG. The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity. 6 isoforms of the human protein are produced by alternative splicing. Isoform 2 is brain specific and is highly expressed in cerebral cortex, putamen, amygdala, hippocampus and caudate nucleus. Weakly expressed in brain stem and cerebellum. Isoform 4 is expressed in skeletal muscle. Protein type: GEFs; Kinase, protein; Protein kinase, CAMK; GEFs, Rac/Rho; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; Trio family. Chromosomal Location of Human Ortholog: 3q21.2. Cellular Component: actin cytoskeleton; cytosol. Molecular Function: ATP binding; GTPase activator activity; guanyl-nucleotide exchange factor activity; metal ion binding; protein binding; protein serine/threonine kinase activity; Rho guanyl-nucleotide exchange factor activity. Biological Process: ephrin receptor signaling pathway; nervous system development; positive regulation of apoptosis; positive regulation of GTPase activity; protein amino acid phosphorylation; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; signal transduction; vesicle-mediated transport. Disease: Coronary Heart Disease, Susceptibility To, 5

0.1 mg

300 USD