product summary
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company name :
MyBioSource
product type :
antibody
product name :
Goat anti-P114-RHO-GEF/ARHGEF18 Antibody
catalog :
MBS420381
quantity :
0.1 mg
price :
300 USD
clonality :
polyclonal
host :
goat
conjugate :
nonconjugated
reactivity :
human, dog
application :
western blot, immunocytochemistry, immunoprecipitation, enzyme immunoassay
more info or order :
product information
catalog number :
MBS420381
products type :
Antibody
products full name :
Goat anti-P114-RHO-GEF/ARHGEF18 Antibody
products short name :
P114-RHO-GEF/ARHGEF18
products name syn :
ARHGEF18; Rho/Rac guanine nucleotide exchange factor (GEF) 18; P114-RHO-GEF; KIAA0521; MGC15913; Rho-specific guanine nucleotide exchange factor p114; P114-RhoGEF; Rho/Rac guanine nucleotide exchange factor 18; ARHGEF18 antibody; Rho/Rac guanine nucleotide exchange factor (GEF) 18 antibody; P114-RHO-GEF antibody; KIAA0521 antibody; MGC15913 antibody; Rho-specific guanine nucleotide exchange factor p114 antibody; P114-RhoGEF antibody; Rho/Rac guanine nucleotide exchange factor 18 antibody; P114-RHO-GEF / ARHGEF18
other names :
rho guanine nucleotide exchange factor 18 isoform a; Rho guanine nucleotide exchange factor 18; rho guanine nucleotide exchange factor 18; Rho/Rac guanine nucleotide exchange factor 18; 114 kDa Rho-specific guanine nucleotide exchange factor; p114-Rho-GEF; p114RhoGEF; Septin-associated RhoGEF; SA-RhoGEF
products gene name :
ARHGEF18
products gene name syn :
P114-RHO-GEF
other gene names :
ARHGEF18; ARHGEF18; P114-RhoGEF; KIAA0521; p114-Rho-GEF; p114RhoGEF; SA-RhoGEF
uniprot entry name :
ARHGI_HUMAN
clonality :
Polyclonal
host :
Goat
reactivity :
Tested: Human, Dog, Medaka; Expected from sequence similarity: Human
sequence length :
1015
sequence :
SAKEDASKEDVIFF
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: This antibody has been successfully used for Caco-2, HCE and MDCK cells as described in the following paper: Terry et al, Nat Cell Biol.2011 Feb;13(2): 159-66.; PMID: 21258369. Immunoprecipitation: This antibody has been successfully used in IP from Caco-2 cells (PMID: 21258369). ICC/IF: This antibody has also been successfully used in ICC/IF for Caco-2 cells (PMID: 21258369).
other info1 :
Immunogen: Peptide with sequence C-SAKEDASKEDVIFF, from the C Terminus of the protein sequence according to NP_056133.2; NP_001124427.1. Epitope: C Terminus
other info2 :
Note: This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).
products references :
Terry SJ, Zihni C, Elbediwy A, Vitiello E, Leefa Chong San IV, Balda MS, Matter K.; Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis.; Nat Cell Biol.2011 Feb;13(2): 159-66.; Pubmed 21258369
ncbi gi num :
41327769
ncbi acc num :
NP_056133.2
ncbi gb acc num :
NM_015318.3
ncbi mol weight :
114,077 Da
ncbi pathways :
Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (1269447); G Alpha (12/13) Signalling Events Pathway (1269579); GPCR Downstream Signaling Pathway (1269574); NRAGE Signals Death Through JNK Pathway (1269448); Regulation Of RhoA Activity Pathway (138070); Rho GTPase Cycle Pathway (1269508); Signal Transduction Pathway (1269379); Signaling By GPCR Pathway (1269543); Signaling By Rho GTPases Pathway (1269507); Signaling By TGF-beta Receptor Complex Pathway (1269523)
ncbi summary :
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]
uniprot summary :
ARHGEF18: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. 2 isoforms of the human protein are produced by alternative splicing. Protein type: GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: apical part of cell; apical plasma membrane; cell junction; cytoplasm; cytoskeleton; cytosol. Molecular Function: guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity. Biological Process: actin cytoskeleton organization and biogenesis; positive regulation of apoptosis; positive regulation of GTPase activity; regulation of cell shape; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; transforming growth factor beta receptor signaling pathway
size1 :
0.1 mg
price1 :
300 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
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