antibody

Goat anti-NDRG1 Antibody

MBS420361

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-NDRG1 Antibody

NDRG1

Goat Anti-NDRG1, Biotinylated Antibody; NDRG1; N-myc downstream regulated gene 1; CAP43,; CMT4D; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5; N-myc downstream regulated gene 1 protein; differentiation-related gene 1 protein; nickel-specific induction protein Cap43; protein reg; NDRG1 antibody; N-myc downstream regulated gene 1 antibody; CAP43; antibody; CMT4D antibody; DRG1 antibody; GC4 antibody; HMSNL antibody; NDR1 antibody; NMSL antibody; PROXY1 antibody; RIT42 antibody; RTP antibody; TARG1 antibody; TDD5 antibody; N-myc downstream regulated gene 1 protein antibody; differentiation-related gene 1 protein antibody; nickel-specific induction protein Cap43 antibody; protein regulated by oxygen-1 antibody; reducing agents and tunicamycin-responsive protein antibody; tunicamycin-responsive protein antibody

protein NDRG1 isoform 1; Protein NDRG1; protein NDRG1; N-myc downstream regulated 1; Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42

NDRG1

NDRG1; NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1; CAP43; DRG1; RTP; DRG-1; RTP

NDRG1_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog

394

GNSAGPKSMEVSC

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cytoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration, 0.5-2ug/ml. Western Blot: Approx 48kDa band observed in Human Kidney, and approx 50kDa band observed in Human Brain (Cerebellum) and Testis lysates (calculated MW of 42.8kDa according to NP_006087.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.

Immunogen: Peptide with sequence GNSAGPKSMEVSC, from the C Terminus of the protein sequence according to NP_006087.2. Epitope: C Terminus

Note: The variants (NP_006087.2; NP_001128714.1) encode the same protein

37655183

NP_006087.2

NM_006096.3

33,650 Da

Direct P53 Effectors Pathway (137939); Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); HIF-1-alpha Transcription Factor Network Pathway (138045); Prostate Cancer Pathway (755440); TP53 Regulates Transcription Of Cell Death Genes Pathway (1383066); TP53 Regulates Transcription Of Several Additional Cell Death Genes Whose Specific Roles In P53-dependent Apoptosis Remain Uncertain Pathway (1383070); Transcriptional Regulation By TP53 Pathway (1269654); Validated Targets Of C-MYC Transcriptional Repression Pathway (169353)

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D). Protein type: Cell development/differentiation; Vesicle. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: cell-cell adherens junction; centrosome; cytoplasm; cytosol; microtubule; microtubule cytoskeleton; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome membrane. Molecular Function: cadherin binding; gamma-tubulin binding; microtubule binding; protein binding; Rab GTPase binding. Biological Process: DNA damage response, signal transduction by p53 class mediator; mast cell activation; myelin maintenance in the peripheral nervous system; negative regulation of cell proliferation; regulation of apoptosis; response to metal ion. Disease: Charcot-marie-tooth Disease, Type 4d

0.1 mg

300 USD