antibody

Goat anti-CARD15/NOD2 (Internal) Antibody

MBS420357

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat

western blot, enzyme immunoassay

Antibody

Goat anti-CARD15/NOD2 (Internal) Antibody

CARD15/NOD2

CARD15; NOD2; caspase recruitment domain family, member 15; ACUG; BLAU; CD; IBD1; NOD2B; PSORAS1; LRR-containing protein; NOD2 protein; caspase recruitment domain protein 15; inflammatory bowel disease protein 1; nucleotide-binding oligomerization domain; CARD15 antibody; NOD2 antibody; caspase recruitment domain family; member 15 antibody; ACUG antibody; BLAU antibody; CD antibody; IBD1 antibody; NOD2B antibody; PSORAS1 antibody; LRR-containing protein antibody; NOD2 protein antibody; caspase recruitment domain protein 15 antibody; inflammatory bowel disease protein 1 antibody; nucleotide-binding oligomerization domain 2 antibody; CARD15 / NOD2 (Internal)

nucleotide-binding oligomerization domain-containing protein 2 isoform 1; Nucleotide-binding oligomerization domain-containing protein 2; nucleotide-binding oligomerization domain-containing protein 2; nucleotide binding oligomerization domain containing 2; Caspase recruitment domain-containing protein 15; Inflammatory bowel disease protein 1

CARD15

NOD2

NOD2; NOD2; CD; ACUG; BLAU; IBD1; NLRC2; NOD2B; CARD15; CLR16.3; PSORAS1; CARD15; IBD1

NOD2_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat

1040

KFRFTDRERHCSPTD

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 100kDa band observed in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 115kDa according to NP_071445.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 60kDa.

Immunogen: Peptide with sequence KFRFTDRERHCSPTD, from the internal region of the protein sequence according to NP_071445.1. Epitope: Internal region

11545912

NP_071445.1

NM_022162.2

22,403 Da

Activated TLR4 Signalling Pathway (1269236); Canonical NF-kappaB Pathway (138030); Cytokine Signaling In Immune System Pathway (1269310); Immune System Pathway (1269170); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Innate Immune System Pathway (1269203); Interleukin-1 Signaling Pathway (1269320); JNK (c-Jun Kinases) Phosphorylation And Activation Mediated By Activated Human TAK1 Pathway (1269212); MAP Kinase Activation In TLR Cascade Pathway (1269211)

This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

NOD2: Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK- gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash. Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1). IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Defects in NOD2 are the cause of sarcoidosis early-onset (EOS). EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early- onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement. 2 isoforms of the human protein are produced by alternative initiation. Chromosomal Location of Human Ortholog: 16q21. Cellular Component: basolateral plasma membrane; cell surface; cytoplasm; cytoskeleton; cytosol; plasma membrane; protein complex; signalosome; vesicle. Molecular Function: actin binding; ATP binding; CARD domain binding; enzyme binding; Hsp70 protein binding; Hsp90 protein binding; muramyl dipeptide binding; peptidoglycan binding; protein binding; protein kinase binding. Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; cytokine production during immune response; cytokine secretion during immune response; defense response; defense response to bacterium; detection of bacterium; detection of biotic stimulus; detection of muramyl dipeptide; innate immune response; JNK cascade; maintenance of gastrointestinal epithelium; positive regulation of B cell activation; positive regulation of dendritic cell antigen processing and presentation; positive regulation of epithelial cell proliferation; positive regulation of gamma-delta T cell activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-10 production; positive regulation of interleukin-17 production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of JNK cascade; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of Notch signaling pathway; positive regulation of oxidoreductase activity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of stress-activated MAPK cascade; positive regulation of T-helper 2 type immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; protein oligomerization; regulation of inflammatory response; response to muramyl dipeptide. Disease: Blau Syndrome; Inflammatory Bowel Disease 1; Psoriatic Arthritis, Susceptibility To; Sarcoidosis, Early-onset

0.1 mg

300 USD