catalog number :
MBS420348
products full name :
Goat anti-PRODH Antibody
products short name :
PRODH
products name syn :
PRODH; proline dehydrogenase (oxidase) 1; HSPOX2; PIG6; PRODH1; PRODH2; SCZD4; TP53I6; FLJ33744; MGC148078; MGC148079; Proline oxidase; mitochondrial; p53 induced protein; proline dehydrogenase (proline oxidase); proline oxidase 2; tumor protein p53 inducible protein 6; Goat Anti-PRODH (aa170-184); PRODH; proline dehydrogenase (oxidase) 1; HSPOX2; PIG6; PRODH1; PRODH2; SCZD4; TP53I6; FLJ33744; MGC148078; MGC148079; Proline oxidase, mitochondrial; p53 induced protein; proline dehydrogenase (proline oxidase); proline oxidase 2; tumor protein p53 inducible protein 6
other names :
proline dehydrogenase (oxidase) 1; Proline dehydrogenase 1, mitochondrial; proline dehydrogenase 1, mitochondrial; proline oxidase 2; p53-induced gene 6 protein; proline oxidase, mitochondrial; tumor protein p53 inducible protein 6; proline dehydrogenase (oxidase) 1; Proline oxidase; Proline oxidase 2; p53-induced gene 6 protein
products gene name :
PRODH
other gene names :
PRODH; PRODH; POX; PIG6; HSPOX2; PRODH1; PRODH2; TP53I6; PIG6; POX2
uniprot entry name :
PROD_HUMAN
reactivity :
Human, Mouse
sequence :
ERDGSGTNKRDKQYQ
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
app notes :
Immunohistochemistry: In paraffin embedded Cerebellum shows mitochondrial staining in smaller neurons. Recommended concentration, 3-6ug/ml. Western Blot: Preliminary experiments gave bands at approx 85kDa and 25kDa in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 68.0kDa according to NP_057419.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Peptide ELISA: antibody detection limit dilution 1:16000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-ERDGSGTNKRDKQYQ, from the internal region of the protein sequence according to NP_057419.2.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS428619
ncbi acc num :
NP_057419.2
ncbi gb acc num :
NM_016335.2
ncbi mol weight :
59,231 Da
ncbi pathways :
Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Amino Acids And Derivatives Pathway 106169!!Proline Catabolism Pathway 106178!!Citrulline Biosynthesis Pathway 138212!!Proline Degradation Pathway 139198!!Superpathway Of Citrulline Metabolism 703106
ncbi summary :
This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010]