catalog number :
MBS420345
products full name :
Goat anti-Wilms tumor 1/WT1 Antibody
products short name :
Wilms tumor 1/WT1
products name syn :
WT1; Wilms tumor 1; GUD; WAGR; WIT-2; WT33; WT1 antibody; Wilms tumor 1 antibody; GUD antibody; WAGR antibody; WIT-2 antibody; WT33 antibody; Wilms tumor 1; Wilms tumor 1 / WT1
other names :
Wilms tumor protein isoform A; Wilms tumor protein; Wilms tumor protein; Wilms tumor 1; WT33
products gene name syn :
WT1
other gene names :
WT1; WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
uniprot entry name :
WT1_HUMAN
reactivity :
Tested: Human; Expected from sequence similarity: Human
sequence :
QDPASTCVPEPASQH
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 55kDa band observed in Human Kidney, Spleen and Testis lysates (calculated MW of 54.3kDa according to NP_000369.3). Recommended concentration: 1-3ug/ml.
other info1 :
Immunogen: Peptide with sequence QDPASTCVPEPASQH, from the N Terminus of the protein sequence according to NP_000369.3; NP_077742.2; NP_077743.2; NP_077744.3. Epitope: N Terminus
other info2 :
Note: This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3).
ncbi acc num :
NP_000369.3
ncbi gb acc num :
NM_000378.4
ncbi mol weight :
33,086 Da
ncbi pathways :
Integrated Pancreatic Cancer Pathway (711360); Regulation Of Telomerase Pathway (137987); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)
ncbi summary :
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]