Goat anti-SOCS3 Antibody | MyBioSource MBS420344 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-SOCS3 Antibody

catalog :

MBS420344

quantity :

0.1 mg

price :

305 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Staining (5ug/ml) of MOLT-4 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

product information

catalog number :

MBS420344

products type :

Antibody

products full name :

Goat anti-SOCS3 Antibody

products short name :

[SOCS3]

products name syn :

[SOCS3; CIS3; Cish3; SSI-3; SOCS-3; suppressor of cytokine signaling 3; STAT induced STAT inhibitor 3; cytokine-induced SH2 protein 3; ATOD4; MGC71791; SSI3; SOCS3 antibody; CIS3 antibody; Cish3 antibody; SSI-3 antibody; SOCS-3 antibody; suppressor of cytokine signaling 3 antibody; STAT induced STAT inhibitor 3 antibody; cytokine-induced SH2 protein 3 antibody; ATOD4 antibody; MGC71791 antibody; SSI3 antibody]

other names :

[suppressor of cytokine signaling 3; Suppressor of cytokine signaling 3; suppressor of cytokine signaling 3; suppressor of cytokine signaling 3; Cytokine-inducible SH2 protein 3; CIS-3; STAT-induced STAT inhibitor 3; SSI-3]

products gene name :

[SOCS3]

other gene names :

[SOCS3; SOCS3; CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3; CIS3; SSI3; SOCS-3; CIS-3; SSI-3]

uniprot entry name :

SOCS3_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human. Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

sequence length :

225

sequence :

PIREFLDQYDAPL

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

storage stability :

Aliquot and store at -20°C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 26kDa band observed in lysates of cell line MOLT4 (calculated MWof 24.8kDa according to NP_003946.3). Recommended concentration: 1-3 ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse, Rat and Pig Liver for this particular batch.

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: Peptide with sequence C-PIREFLDQYDAPL, from the C Terminus of the protein sequence according to NP_003946.3. Epitope: C Terminus

other info2 :

Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7009

ncbi gi num :

45439352

ncbi acc num :

NP_003946.3

ncbi gb acc num :

NM_003955.4

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Adaptive Immune System Pathway (1269171); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (1269193); Class I MHC Mediated Antigen Processing Presentation Pathway (1269192); Cytokine Signaling In Immune System Pathway (1269310); ECS Complex Pathway (413455); ECS Complex Pathway (468394)

ncbi summary :

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008]

uniprot summary :

SOCS3: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2. Interacts with FGFR3. Interacts with INSR. Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. Protein type: Inhibitor. Chromosomal Location of Human Ortholog: 17q25.3. Cellular Component: cytoplasm; cytosol. Molecular Function: protein binding; protein kinase inhibitor activity. Biological Process: aging; cytokine and chemokine mediated signaling pathway; JAK-STAT cascade; negative regulation of apoptosis; negative regulation of inflammatory response; negative regulation of insulin receptor signaling pathway; negative regulation of JAK-STAT cascade; negative regulation of protein kinase activity; organ regeneration; positive regulation of cell differentiation; positive regulation of tyrosine phosphorylation of Stat3 protein; protein ubiquitination; regulation of growth; response to drug; response to estradiol stimulus; response to food; response to gamma radiation; response to glucocorticoid stimulus; response to heat; response to hypoxia; response to insulin stimulus; response to lipopolysaccharide; response to progesterone stimulus. Disease: Dermatitis, Atopic, 4

size1 :

0.1 mg

price1 :

305 USD

more info or order :

MyBioSource product webpage