antibody

Goat anti-SF3B4/SAP49 Antibody

MBS420319

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, enzyme immunoassay

Antibody

Goat anti-SF3B4/SAP49 Antibody

SF3B4/SAP49

SF3B4; SAP49; splicing factor 3b, subunit 4, 49kD; SF3B49; MGC10828; spliceosome-associated protein (U2 snRNP); spliceosomal protein; splicing factor 3b, subunit 4; SF3b49; pre-mRNA splicing factor SF3b 49 kDa subunit; splicing factor 3b, subunit 4; SF3B4 antibody; SAP49 antibody; splicing factor 3b; subunit 4; 49kD antibody; SF3B49 antibody; MGC10828 antibody; spliceosome-associated protein (U2 snRNP) antibody; spliceosomal protein antibody; splicing factor 3b; subunit 4 antibody; SF3b49 antibody; pre-mRNA splicing factor SF3b 49 kDa subunit antibody; splicing factor 3b; subunit 4 antibody; SF3B4 / SAP49

splicing factor 3B subunit 4; Splicing factor 3B subunit 4; splicing factor 3B subunit 4; splicing factor 3b subunit 4; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SF3b50; Spliceosome-associated protein 49; SAP 49

SF3B4

SAP49

SF3B4; SF3B4; AFD1; Hsh49; SAP49; SF3b49; SAP49; SAP 49

SF3B4_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

424

AAGPISERNQDAT

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 48kDa band observed in Human HeLa lysates (calculated MW of 44.4kDa according to NP005841.1). Recommended concentration: 0.1-0.3ug/ml.

Immunogen: Peptide with sequence AAGPISERNQDAT-C, from the N Terminus of the protein sequence according to NP_005841.1. Epitope: N Terminus

5032069

NP_005841.1

NM_005850.4

44,386 Da

Gene Expression Pathway (1269649); Processing Of Capped Intron-Containing Pre-mRNA Pathway (1269688); Spliceosome Pathway (125136); Spliceosome Pathway (124832); Spliceosome, U2-snRNP Pathway (413436); Spliceosome, U2-snRNP Pathway (890599); MRNA Splicing Pathway (1269689); MRNA Splicing - Major Pathway (1269690); MRNA Splicing - Minor Pathway (1269691); MRNA Processing Pathway (198843)

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq, Jul 2008]

SF3B4: subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Protein type: RNA splicing; RNA-binding; Spliceosome. Chromosomal Location of Human Ortholog: 1q21.2. Cellular Component: nucleoplasm; spliceosome; U12-dependent spliceosome. Molecular Function: nucleotide binding; protein binding. Biological Process: mRNA processing; nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; RNA splicing, via transesterification reactions. Disease: Acrofacial Dysostosis 1, Nager Type

0.1 mg

300 USD