antibody

Goat anti-AKAP9/AKAP450/CG-NAP Antibody

MBS420313

0.1 mg

300 USD

polyclonal

goat

nonconjugated

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-AKAP9/AKAP450/CG-NAP Antibody

AKAP9/AKAP450/CG-NAP

Goat Anti-AKAP9 / YOTIAO Antibody; AKAP9; CG-NAP; PRKA9; YOTIAO; AKAP350; HYPERION; KIAA0803; A kinase (PRKA) anchor protein (yotiao) 9; AKAP120-like protein; kinase N-associated protein; A-kinase anchoring protein 450; A-kinase anchor protein, 350kDa; protein kinase A anchoring protein 9; AKAP9 antibody; CG-NAP antibody; PRKA9 antibody; YOTIAO antibody; AKAP350 antibody; HYPERION antibody; KIAA0803 antibody; A kinase (PRKA) anchor protein (yotiao) 9 antibody; AKAP120-like protein antibody; kinase N-associated protein antibody; A-kinase anchoring protein 450 antibody; A-kinase anchor protein; 350kDa antibody; protein kinase A anchoring protein 9 antibody; centrosome- and golgi-localized protein antibody; AKAP450 antibody; MU-RMS-40.16A antibody; A-kinase anchor protein 9 antibody; AKAP9-BRAF fusion protein antibody; AKAP9 / AKAP450 / CG-NAP

A-kinase anchor protein 9 isoform 2; A-kinase anchor protein 9; A-kinase anchor protein 9; A-kinase anchoring protein 9; A-kinase anchor protein 350 kDa; AKAP 350; hgAKAP 350; A-kinase anchor protein 450 kDa; AKAP 450; AKAP 120-like protein; Centrosome- and Golgi-localized PKN-associated protein

AKAP9

AKAP450; CG-NAP

AKAP9; AKAP9; LQT11; PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; PPP1R45; HYPERION; MU-RMS-40.16A; AKAP350; AKAP450; KIAA0803; PRKA9

AKAP9_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse

3907

SGSTTQFHAGMR

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: The staining pattern observed was identical to that obtained with a rabbit polyclonal antibody specific to AKAP350A (to be submitted to a scientific journal). Western Blot: Approx 450kDa band observed in lysates of cell line HepG2 (calculated MW of 454kDa according to NP_ 671700.1). Recommended concentration: 1-3ug/ml.

Immunogen: Peptide with sequence C-SGSTTQFHAGMR, from the C Terminus of the protein sequence according to NP_005742.4; NP_671714.1. Epitope: C Terminus

Note: This antibody is expected to recognise isoforms 2 and 3 (NP_005742.4; NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.

22538387

NP_005742.4

NM_005751.4

455,423 Da

ARMS-mediated Activation Pathway (1269471); AURKA Activation By TPX2 Pathway (1383018); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (1268798); Anchoring Of The Basal Body To The Plasma Membrane Pathway (1268847); Assembly Of The Primary Cilium Pathway (1268846); Axon Guidance Pathway (1270303); CREB Phosphorylation Through The Activation Of CaMKII Pathway (1268805); CREB Phosphorylation Through The Activation Of Ras Pathway (1268801); Cardiac Conduction Pathway (1339115); Cell Cycle Pathway (1269741)

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in at least two isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq, Aug 2008]

AKAP9: Binds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations. Defects in AKAP9 are the cause of long QT syndrome type 11 (LQT11). Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 7q21-q22. Cellular Component: centrosome; cis-Golgi network; cytoskeleton; cytosol; Golgi apparatus; Golgi stack; intracellular membrane-bound organelle; voltage-gated potassium channel complex. Molecular Function: potassium channel regulator activity; protein binding; protein complex scaffold; Ras guanyl-nucleotide exchange factor activity; receptor binding. Biological Process: G2/M transition of mitotic cell cycle; MAPKKK cascade; microtubule nucleation; positive regulation of GTPase activity; positive regulation of peptidyl-serine phosphorylation; signal transduction; synaptic transmission; transport. Disease: Long Qt Syndrome 11

0.1 mg

300 USD