catalog number :
MBS420309
products full name :
Goat anti-VPS25 Antibody
products short name :
VPS25
products name syn :
VPS25; vacuolar protein sorting 25 (yeast); HGNC:28122; DERP9; EAP20; MGC10540; vacuolar protein sorting 25; vacuolar protein sorting 25 homolog (S. cerevisiae); FAP20; VPS25; vacuolar protein sorting 25 (yeast); HGNC:28122; DERP9; EAP20; MGC10540; vacuolar protein sorting 25; vacuolar protein sorting 25 homolog (S. cerevisiae); FAP20
other names :
vacuolar protein-sorting-associated protein 25; Vacuolar protein-sorting-associated protein 25; vacuolar protein-sorting-associated protein 25; ESCRT-II complex subunit VPS25; ELL-associated protein of 20 kDa; dermal papilla-derived protein 9; vacuolar protein sorting 25 homolog (S. cerevisiae); Dermal papilla-derived protein 9; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25
products gene name :
VPS25
other gene names :
VPS25; VPS25; DERP9; EAP20; FAP20; DERP9; EAP20; hVps25
uniprot entry name :
VPS25_HUMAN
reactivity :
Human, Mouse, Rat, Dog
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
app notes :
Immunohistochemistry: In paraffin embedded human kidney, shows granular cytoplasmic staining of endothelial cells. Recommended concentration, 10ug/ml. Western Blot: Approx 20-22kDa kDa band observed in human duodenum, kidney and liver lysates (calculated MW of 20.7kDa according to NP_115729.1). Recommended concentration: 0.5-1.5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:1000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-QPNVDTRQKQ, from the internal region of the protein sequence according to NP_115729.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS426294
ncbi acc num :
NP_115729.1
ncbi gb acc num :
NM_032353.3
ncbi mol weight :
20,748 Da
ncbi pathways :
ESCRT-II Complex Pathway 413474!!ESCRT-II Complex Pathway 890625!!Endocytosis Pathway 102279!!Endocytosis Pathway 102181!!Endosomal Sorting Complex Required For Transport (ESCRT) Pathway 205244!!Membrane Trafficking Pathway 106160
ncbi summary :
This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]