antibody

Goat anti-PAX3 Antibody

MBS420302

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat, cow

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-PAX3 Antibody

PAX3

PAX3; WS1; CDHS; HUP2; paired box gene 3 (Waardenburg syndrome 1); paired domain gene 3; paired box homeotic gene 3 (Waardenburg syndrome 1); paired box 3; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box homeotic gene 3; paired domain gene HuP2; PAX3; WS1; CDHS; HUP2; paired box gene 3 (Waardenburg syndrome 1); paired domain gene 3; paired box homeotic gene 3 (Waardenburg syndrome 1); paired box 3; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box homeotic gene 3; paired domain gene HuP2

paired box protein Pax-3 isoform PAX3a; Paired box protein Pax-3; paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; paired box homeotic gene 3; paired box 3; HuP2

PAX3

PAX3; PAX3; WS1; WS3; CDHS; HUP2; HUP2

PAX3_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Cow

215

TTLAGAVPRMM

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Immunohistochemistry: In paraffin embedded Human Oesophagus shows nuclear staining of cells in the Squamous Epithelium. Recommended concentration, 3ug/ml. Data obtained using a previous batch. Western Blot: Approx 45kDa band observed in lysates of Human Brain (Cerebellum), Duodenum, Kidney and Skin, in Rat Duodenum and Stomach lysates, in lysates of cell lines A431 and Hela, and in nuclear lysates of Jurkat (calculated MW of 45.2kDa according to NP_852125.1 and of 44.8kDa according to NP_852126.1). Recommended concentration: 0.3-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.

Epitope: N Terminus. Immunogen: Peptide with sequence TTLAGAVPRMM-C, from the N Terminus of the protein sequence according to NP_000429.2; NP_039230.1; NP_852122.1.1 NP_852123.1; NP_852124.1; NP_852125.1; NP_852126.1. Corresponding Immunizing Peptide: Catalog #MBS427999

Important Note: This antibody is expected to recognise all 7 reported human isoforms (as represented by NP_000429.2; NP_039230.1; NP_852122.1;.1 NP_852123.1; NP_852124.1; NP_852125.1 and NP_852126.1).

Hsieh MJ, Yao YL, Lai IL, Yang WM.; Transcriptional repression activity of PAX3 is modulated by competition between corepressor KAP1 and heterochromatin protein 1.; Biochem Biophys Res Commun. 2006 Oct 20; 349(2):573-81.; Pubmed 16945326

6654638

NP_000429.2

NM_000438.5

55,975 Da

Neural Crest Differentiation Pathway 672460!!Regulation Of Retinoblastoma Protein Pathway 137916!!Transcriptional Misregulation In Cancer Pathway 523016!!Transcriptional Misregulation In Cancer Pathway 522987

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]

0.1 mg

290 USD