catalog number :
MBS420279
products full name :
Goat anti-Monoglyceride Lipase Antibody
products short name :
Monoglyceride Lipase
products name syn :
monoglyceride lipase; HU-K5; MGLL; MGL; lysophospholipase-like; monoglyceride lipase; HU-K5; MGLL; MGL; lysophospholipase-like
other names :
monoglyceride lipase isoform 1; Monoglyceride lipase; monoglyceride lipase; monoacylglycerol lipase; lysophospholipase homolog; monoglyceride lipase; HU-K5; Lysophospholipase homolog; Lysophospholipase-like; Monoacylglycerol lipase; MAGL
products gene name :
MGLL
other gene names :
MGLL; MGLL; MGL; HUK5; MAGL; HU-K5; MGL; MAGL
uniprot entry name :
MGLL_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Pig, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx. 30kDa band observed in Human Adipose and Brain (Frontal Cortex) lysates (calculated MW of 33.3kDa according to NP_001003794.1). In transfected HEK293 transiently expressing MGLL a band of approx. 36kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: Internal Region (Near N Terminus). Immunogen: Peptide with sequence C-QDLPHLVNADGQY, from the internal region (near N Terminus) of the protein sequence according to NP_009214.1; NP_001003794.1. Corresponding Immunizing Peptide: Catalog #MBS426275
other info2 :
Important Note: This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1).
ncbi acc num :
NP_009214.1
ncbi gb acc num :
NM_007283.6
ncbi mol weight :
31,149 Da
ncbi pathways :
Acyl Chain Remodeling Of DAG And TAG Pathway 645315!!Acylglycerol Degradation Pathway 413388!!Acylglycerol Degradation Pathway 468272!!Arachidonate Production From DAG Pathway 161024!!Effects Of PIP2 Hydrolysis Pathway 106044!!G Alpha (q) Signalling Events Pathway 106043!!GPCR Downstream Signaling Pathway 119548!!Gastrin-CREB Signalling Pathway Via PKC And MAPK 645295!!Glycerolipid Metabolism Pathway 82986!!Glycerolipid Metabolism Pathway 361
ncbi summary :
This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]