catalog number :
MBS420270
products full name :
Goat anti-DDX5 / p68 RNA helicase Antibody
products short name :
DDX5/p68 RNA helicase
products name syn :
DDX5; P68; HLR1; G17P1; HUMP68; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase; 68kDa); RNA-dependent ATPase; DEAD/H box-5 (RNA helicase; 68kD); DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase; 68kD); DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DKFZp434E109; DKFZp686J01190; ATP-dependent RNA helicase DDX5; DEAD box-5; DDX5; P68; HLR1; G17P1; HUMP68; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kDa); RNA-dependent ATPase; DEAD/H box-5 (RNA helicase, 68kD); DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DKFZp434E109; DKFZp686J01190; ATP-dependent RNA helicase DDX5; DEAD box-5
other names :
probable ATP-dependent RNA helicase DDX5; Probable ATP-dependent RNA helicase DDX5; probable ATP-dependent RNA helicase DDX5; DEAD box-5; RNA helicase p68; DEAD box protein 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD box protein 5; RNA helicase p68
products gene name :
DDX5
other gene names :
DDX5; DDX5; p68; HLR1; G17P1; HUMP68; G17P1; HELR; HLR1
uniprot entry name :
DDX5_HUMAN
reactivity :
Human, Mouse, Rat, Dog
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
app notes :
Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining of the secretory cells in the glands. Recommended concentration, 2-5ug/ml. Data obtained using a previous batch. Western Blot: Approx 70kDa band observed in nuclear lysates of cell lines NIH3T3, A431, HeLa and Jurkat (calculated MW of 69.1kDa according to NP_004387.1). Recommended concentration: 0.3-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000. Immunofluorescence: A customer has reported successful staining at a dilution of 1:100 on HT-1080 cells with a Rhodamine conjugated secondary antibody. Data obtained using a previous batch.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-PMIGYPMPTGYSQ, from the C Terminus of the protein sequence according to NP_004387.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425805
ncbi acc num :
NP_004387.1
ncbi gb acc num :
NM_004396.3
ncbi mol weight :
60,563 Da
ncbi pathways :
Direct P53 Effectors Pathway 137939!!Proteoglycans In Cancer Pathway 782000!!Proteoglycans In Cancer Pathway 782054!!Spliceosome Pathway 125136!!Spliceosome Pathway 124832!!Transcriptional Misregulation In Cancer Pathway 523016!!Transcriptional Misregulation In Cancer Pathway 522987
ncbi summary :
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq, Jul 2008]