catalog number :
MBS420248
products full name :
Goat anti-CBX3 / HP1 Gamma Antibody
products short name :
CBX3/HP1 Gamma
products name syn :
CBX3; HP1-GAMMA; chromobox homolog 3 (HP1 gamma homolog; Drosophila); HGNC:1553; HECH; HP1Hs-gamma; HP1 gamma homolog; chromobox homolog 3; chromobox homolog 3 (Drosophila HP1 gamma); heterochromatin protein HP1 gamma; heterochromatin-like protein 1; CBX3; HP1-GAMMA; chromobox homolog 3 (HP1 gamma homolog, Drosophila); HGNC:1553; HECH; HP1Hs-gamma; HP1 gamma homolog; chromobox homolog 3; chromobox homolog 3 (Drosophila HP1 gamma); heterochromatin protein HP1 gamma; heterochromatin-like protein 1
other names :
chromobox protein homolog 3; Chromobox protein homolog 3; chromobox protein homolog 3; HP1 gamma homolog; modifier 2 protein; heterochromatin-like protein 1; heterochromatin protein HP1 gamma; heterochromatin protein 1 homolog gamma; chromobox homolog 3 (HP1 gamma homolog, Drosophila); chromobox homolog 3; HECH; Heterochromatin protein 1 homolog gamma; HP1 gamma; Modifier 2 protein
products gene name :
CBX3
other gene names :
CBX3; CBX3; HECH; HP1-GAMMA; HP1Hs-gamma; HP1 gamma
uniprot entry name :
CBX3_HUMAN
reactivity :
Human, Mouse, Rat, Dog
sequence :
EAFLNSQKAGKEKD
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
app notes :
Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3ug/ml. Western Blot: Approx. 20kDa band observed in lysates of cell lines A431, HeLa, HepG2, NIH3T3 and NSO (calculated MW of 20.8kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-EAFLNSQKAGKEKD, from the internal region of the protein sequence according to NP_009207.2; NP_057671.2. Corresponding Immunizing Peptide: Catalog #MBS426056
other info2 :
Important Note: No cross-reactivity expected with HP1-alpha and HP1-beta. NP_009207.2 and NP_057671.2 represent same protein.
ncbi acc num :
NP_009207.2
ncbi gb acc num :
NM_007276.4
ncbi mol weight :
20,811 Da
ncbi pathways :
Diurnally Regulated Genes With Circadian Orthologs Pathway 198813!!Gene Expression Pathway 105937!!RNA Polymerase I Chain Elongation Pathway 106555!!RNA Polymerase I Transcription Pathway 106550!!RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway 160948
ncbi summary :
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011]