antibody

Goat anti-KAP1/TRIM28 Antibody

MBS420240

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse

western blot, immunohistochemistry, immunocytochemistry, enzyme immunoassay, chromatin immunoprecipitation

Antibody

Goat anti-KAP1/TRIM28 Antibody

KAP1/TRIM28

Goat Anti-KAP1 Antibody; TRIM28; KAP1; TF1B; RNF96; TIF1B; tripartite motif-containing 28; KRAB-associated protein 1; nuclear corepressor KAP-1; transcriptional intermediary factor 1-beta; FLJ29029; KRAB-associated protein 1; tripartite motif-containing 28 protein; TRIM28 antibody; KAP1 antibody; TF1B antibody; RNF96 antibody; TIF1B antibody; tripartite motif-containing 28 antibody; KRAB-associated protein 1 antibody; nuclear corepressor KAP-1 antibody; transcriptional intermediary factor 1-beta antibody; FLJ29029 antibody; KRAB-associated protein 1 antibody; tripartite motif-containing 28 protein antibody; KAP1 / TRIM28

transcription intermediary factor 1-beta; Transcription intermediary factor 1-beta; transcription intermediary factor 1-beta; tripartite motif containing 28; E3 SUMO-protein ligase TRIM28 (EC:6.3.2.-); KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein 28

TRIM28

KAP1

TRIM28; TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157; KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1

TIF1B_HUMAN

Polyclonal

Goat

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse

835

SSQELSGGPGDGP

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)

Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration, 2-4ug/ml. This product has been successfully used on Human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol.2010 Mar;17(3): 821-8. (PMID: 19898899). Recommended concentration: 5ug/ml. Western Blot: Approx 120kDa band observed in lysates of cell line HepG2 (calculated MW of 88.5kDa according to NP_005753.1). The higher observed MW is explained by sumoylated protein. Recommended concentration: 1-3ug/ml. Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5ug/ml (personal communication). Chromatin immunoprecipitation: This antibody has been successfully used in ChIP for K562 cells as described in the following paper: Frietze et al, PLoS One.2010 Dec 8;5(12): e15082.; PMID: 21170338.

Immunogen: Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1. Epitope: C Terminus

5032179

NP_005753.1

NM_005762.2

79,474 Da

C-MYB Transcription Factor Network Pathway (138073); Corticotropin-releasing Hormone Pathway (920957); E2F Transcription Factor Network Pathway (137934); Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); P53 Pathway (138067)

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Protein type: Ligase; Transcription, coactivator/corepressor; Ubiquitin ligase; Protein kinase, atypical; Nuclear receptor co-regulator; Ubiquitin conjugating system; Kinase, protein; EC 6.3.2.-; ATYPICAL group; TIF1 family. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: nuclear heterochromatin; nucleoplasm; nucleus. Molecular Function: DNA binding; ligase activity; protein binding; protein kinase activity; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding. Biological Process: chromatin modification; convergent extension involved in axis elongation; DNA methylation during embryonic development; DNA repair; embryo implantation; epithelial to mesenchymal transition; innate immune response; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of DNA repair; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein oligomerization; protein sumoylation; protein ubiquitination; Ras protein signal transduction; transcription initiation from RNA polymerase II promoter

0.1 mg

300 USD