catalog number :
MBS420238
products full name :
Goat anti-PTCH (Internal) Antibody
products short name :
PTCH
products name syn :
PTCH; patched homolog (Drosophila); RP11-435O5.3; BCNS; FLJ42602; HPE7; NBCCS; PTC; PTC1; PTCH1; PTCH protein +12b; PTCH protein +4 1; PTCH protein -10; patched; patched (Drosophila) homolog; Goat Anti-PTCH (C Terminus); PTCH; patched homolog (Drosophila); RP11-435O5.3; BCNS; FLJ42602; HPE7; NBCCS; PTC; PTC1; PTCH1; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; patched; patched (Drosophila) homolog
other names :
protein patched homolog 1 isoform L; Protein patched homolog 1; protein patched homolog 1; PTCH protein +4'; PTCH protein -10; PTCH protein +12b; PTCH protein -3,4,5; patched 1
products gene name :
PTCH
other gene names :
PTCH1; PTCH1; PTC; BCNS; HPE7; PTC1; PTCH; NBCCS; PTCH11; PTCH; PTC; PTC1
uniprot entry name :
PTC1_HUMAN
reactivity :
Human, Dog, Pig, Cow, Mouse
sequence :
HPESRHHPPSNPRQQ
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
app notes :
Western Blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 161kDa according to NP_000255.1). Recommended concentration: 1-3ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF for C3H10T1/2 cells and mouse embryonic primary cells.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-HPESRHHPPSNPRQQ, from the internal region of the protein sequence according to NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1. Corresponding Immunizing Peptide: Catalog #MBS426691
other info2 :
Important Note: This antibody is expected to recognise all four reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1). Reported variants represent identical protein (NP_001077075.1; NP_001077076.1; NP_001077073.1; NP_001077074.1).
ncbi acc num :
NP_000255.2
ncbi gb acc num :
NM_000264.3
ncbi mol weight :
144,356 Da
ncbi pathways :
Basal Cell Carcinoma Pathway 83113!!Basal Cell Carcinoma Pathway 525!!Class B/2 (Secretin Family Receptors) Pathway 106378!!Endochondral Ossification Pathway 198812!!GPCR Ligand Binding Pathway 161020!!Glypican 3 Network Pathway 138084!!Hedgehog Signaling Pathway 198835!!Hedgehog Signaling Events Mediated By Gli Proteins Pathway 137912!!Hedgehog Signaling Pathway 83063!!Hedgehog Signaling Pathway 474
ncbi summary :
This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq, Jul 2008]