antibody

Goat anti-SLIT2 Antibody

MBS420237

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-SLIT2 Antibody

SLIT2

SLIT2; slit homolog 2 (Drosophila); HGNC: 11086; SLIL3; Slit-2; SLIT2 antibody; slit homolog 2 (Drosophila) antibody; HGNC: 11086 antibody; SLIL3 antibody; Slit-2 antibody

slit homolog 2 protein isoform 1; Slit homolog 2 protein; slit homolog 2 protein; slit guidance ligand 2

SLIT2

SLIT2; SLIT2; SLIL3; Slit-2; SLIL3; Slit-2

SLIT2_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog

1529

DDCQDNKCKNGAH

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 1000. Immunohistochemistry: In paraffin embedded Human Spinal Cord shows staining of the cellular membrane in the bundles of sensory fibres. Recommended concentration, 4-8ug/ml.

Immunogen: Peptide with sequence DDCQDNKCKNGAH, from the internal region of the protein sequence according to NP_004778.1. Epitope: Internal region

4759146

NP_004778.1

NM_004787.3

168,893 Da

Activation Of Rac Pathway (1270321); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (1270303); Developmental Biology Pathway (1270302); Glypican 1 Network Pathway (138010); Inactivation Of Cdc42 And Rac Pathway (1270320); Netrin-1 Signaling Pathway (1270314); Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway (1270319); Role Of Abl In Robo-Slit Signaling Pathway (1270322)

This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

SLIT2: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth- stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post- crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4p15.2. Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; membrane; plasma membrane; proteinaceous extracellular matrix. Molecular Function: calcium ion binding; GTPase inhibitor activity; heparan sulfate proteoglycan binding; heparin binding; identical protein binding; laminin-1 binding; protein binding; protein homodimerization activity; proteoglycan binding; Roundabout binding. Biological Process: axon extension involved in axon guidance; axon guidance; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; cellular response to hormone stimulus; chemorepulsion involved in embryonic olfactory bulb interneuron migration; chemorepulsion involved in postnatal olfactory bulb interneuron migration; corticospinal neuron axon guidance through the spinal cord; induction of negative chemotaxis; motor axon guidance; negative chemotaxis; negative regulation of actin filament polymerization; negative regulation of cell growth; negative regulation of cell migration; negative regulation of inflammatory response; negative regulation of leukocyte chemotaxis; negative regulation of protein amino acid phosphorylation; negative regulation of small GTPase mediated signal transduction; negative regulation of smooth muscle cell migration; negative regulation of vascular permeability; positive regulation of apoptosis; positive regulation of axonogenesis; response to cortisol stimulus; response to nutrient levels; retinal ganglion cell axon guidance; spinal cord development; ureteric bud development

0.1 mg

300 USD