antibody

Goat anti-Histamine Receptor H2 Antibody

MBS420227

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

western blot, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Goat anti-Histamine Receptor H2 Antibody

Histamine Receptor H2

HRH2; histamine receptor H2; HGNC:5183; H2R; gastric receptor 1; OTTHUMP00000161242; Goat Anti-Histamine Receptor H2 (aa309-323); HRH2; histamine receptor H2; HGNC:5183; H2R; gastric receptor 1; OTTHUMP00000161242

histamine H2 receptor isoform 2; Histamine H2 receptor; histamine H2 receptor; HH2R; gastric receptor 1; gastric receptor I; histamine receptor H2; Gastric receptor I

HRH2

HRH2; HRH2; H2R; H2R; HH2R

HRH2_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Dog

359

QEEKPLKLQVWSGTE

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)

Immunohistochemistry: This antibody was successfully used on coronal rat brain slices as reported in Peng et al, Neurosci Lett. 2013 Apr 29;541:87-92. PMID:23466693. Western Blot: Preliminary experiments gave bands at approx 55kDa and a stronger 28kDa in Human Colon and Duodenum lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 40.1kDa according to NP_071640.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Peptide ELISA: antibody detection limit dilution 1:128000.

Epitope: C Terminus. Immunogen: Peptide with sequence C-QEEKPLKLQVWSGTE, from the C Terminus of the protein sequence according to NP_071640.1; NP_001124527.1. Corresponding Immunizing Peptide: Catalog #MBS428182

Important Note: This antibody is expected to recognise both reported isoforms.

13435405

NP_071640.1

NM_022304.2

44,541 Da

Amine Ligand-binding Receptors Pathway 106363!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!G Alpha (s) Signalling Events Pathway 119549!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!GPCRs, Class A Rhodopsin-like Pathway 198886!!Gastric Acid Secretion Pathway 154409!!Gastric Acid Secretion Pathway 154383

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

0.1 mg

290 USD