Goat anti-ELMO1 Antibody | MyBioSource MBS420226 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-ELMO1 Antibody

catalog :

MBS420226

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow, zebrafish

application :

western blot, immunoprecipitation, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420226

products type :

Antibody

products full name :

Goat anti-ELMO1 Antibody

products short name :

ELMO1

products name syn :

ELMO1; engulfment and cell motility 1 (ced-12 homolog, C. elegans); CED12; KIAA0281; ced-12 homolog 1; engulfment and cell motility 1; CED-12; ELMO-1; MGC126406; OTTHUMP00000207056; OTTHUMP00000207060; ELMO1 antibody; engulfment and cell motility 1 (ced-12 homolog; C. elegans) antibody; CED12 antibody; KIAA0281 antibody; ced-12 homolog 1 antibody; engulfment and cell motility 1 antibody; CED-12 antibody; ELMO-1 antibody; MGC126406 antibody; OTTHUMP00000207056 antibody; OTTHUMP00000207060 antibody

other names :

engulfment and cell motility protein 1 isoform 1; Engulfment and cell motility protein 1; engulfment and cell motility protein 1; engulfment and cell motility 1; Protein ced-12 homolog

products gene name :

ELMO1

other gene names :

ELMO1; ELMO1; CED12; CED-12; ELMO-1; KIAA0281

uniprot entry name :

ELMO1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Mouse, Rat, Cow, Zebrafish; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Zebrafish

sequence length :

727

sequence :

PKEPSNYDFVYDCN

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8). Recommended concentration: 0.1-0.3ug/ml. Immunoprecipitation: This antibody has been successfully used in IP from BAEC cells as described in the following paper: Epting et al, Circ Res.2010 Jul 9;107(1): 45-55; PMID: 20466982. IP; An anonymous customer found positive results in IP on Human promyelocytic leukemia cells (HL60).

other info1 :

Immunogen: Peptide with sequence PKEPSNYDFVYDCN, from the C Terminus of the protein sequence according to NP_055615.8; NP_569709.1; NP_001034548.1. Epitope: C Terminus

other info2 :

Note: This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform.

ncbi gi num :

18765700

ncbi acc num :

NP_055615.8

ncbi gb acc num :

NM_014800.10

ncbi mol weight :

49,915 Da

ncbi pathways :

Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Disease Pathway (1268854); Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway (1269279); HIV Infection Pathway (1269057); Host Interactions Of HIV Factors Pathway (1269091); Immune System Pathway (1269170); Infectious Disease Pathway (1269056)

ncbi summary :

This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

uniprot summary :

ELMO1: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 7p14.1. Cellular Component: cytoplasm; cytosol; membrane; plasma membrane. Molecular Function: guanyl-nucleotide exchange factor activity; protein binding; SH3 domain binding. Biological Process: actin cytoskeleton organization and biogenesis; apoptosis; cell migration; cell motility; phagocytosis, engulfment; positive regulation of GTPase activity; Rac protein signal transduction; regulation of defense response to virus by virus; vascular endothelial growth factor receptor signaling pathway

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage