Goat anti-Pyruvate dehydrogenase/PDHX Antibody | MyBioSource MBS420192 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-Pyruvate dehydrogenase/PDHX Antibody

catalog :

MBS420192

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, dog

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420192

products type :

Antibody

products full name :

Goat anti-Pyruvate dehydrogenase/PDHX Antibody

products short name :

Pyruvate dehydrogenase/PDHX

products name syn :

PDHX; pyruvate dehydrogenase complex, component X; E3BP; OPDX; PDX1; proX; DLDBP; E3-binding protein; pyruvate dehydrogenase complex, lipoyl-containing component X; dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; pyruvate; PDHX antibody; pyruvate dehydrogenase complex; component X antibody; E3BP antibody; OPDX antibody; PDX1 antibody; proX antibody; DLDBP antibody; E3-binding protein antibody; pyruvate dehydrogenase complex; lipoyl-containing component X antibody; dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex antibody; pyruvate dehydrogenase complex; E3-binding protein subunit antibody; pyruvate dehydrogenase protein X component; mitochondrial antibody; Pyruvate dehydrogenase; Pyruvate dehydrogenase / PDHX

other names :

pyruvate dehydrogenase protein X component, mitochondrial isoform 1; Pyruvate dehydrogenase protein X component, mitochondrial; pyruvate dehydrogenase protein X component, mitochondrial; pyruvate dehydrogenase complex component X; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; E3-binding protein; E3BP; Lipoyl-containing pyruvate dehydrogenase complex component X; proX

products gene name :

PDHX

other gene names :

PDHX; PDHX; E3BP; OPDX; PDX1; proX; DLDBP; PDX1; E3BP

uniprot entry name :

ODPX_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog

sequence length :

501

sequence :

KSFKANLENPIRLA

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 50kDa band observed in Human Pancreas lysate (calculated MW of 54.2kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx.50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-1ug/ml.

other info1 :

Immunogen: Peptide with sequence C-KSFKANLENPIRLA, from the C Terminus of the protein sequence according to NP_003468.2; NP_001128496.1; NP_001159630.1. Epitope: C Terminus

other info2 :

Note: This antibody is expected to recognise all reported isoforms.

ncbi gi num :

203098753

ncbi acc num :

NP_003468.2

ncbi gb acc num :

NM_003477.2

ncbi mol weight :

51,460 Da

ncbi pathways :

Glycolysis And Gluconeogenesis Pathway (198814); Glyoxylate Metabolism And Glycine Degradation Pathway (1270180); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Amino Acids And Derivatives Pathway (1270158); Pyruvate Metabolism Pathway (1270123); Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway (1270122); Regulation Of Pyruvate Dehydrogenase (PDH) Complex Pathway (1270124); Signal Transduction Pathway (1269379); Signaling By Retinoic Acid Pathway (1269630)

ncbi summary :

The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP). This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage