Goat anti-NANOG Antibody | MyBioSource MBS420182 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-NANOG Antibody

catalog :

MBS420182

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, dog

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420182

products type :

Antibody

products full name :

Goat anti-NANOG Antibody

products short name :

NANOG

products name syn :

Goat Anti-NANOG (C Terminus) Antibody; NANOG; Nanog homeobox; HGNC: 20857; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG antibody; Nanog homeobox antibody; HGNC: 20857 antibody; homeobox transcription factor Nanog antibody; homeobox transcription factor Nanog-delta 48 antibody

other names :

homeobox protein NANOG isoform 1; Homeobox protein NANOG; homeobox protein NANOG; Nanog homeobox; Homeobox transcription factor Nanog; hNanog

products gene name :

NANOG

other gene names :

NANOG; NANOG; hNanog

uniprot entry name :

NANOG_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Pig; Expected from sequence similarity: Human, Dog, Pig

sequence length :

305

sequence :

QNQRMKSKRWQKNN

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA (EIA)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: This antibody was successfully used on Human Oocytes in Vassena et al, Hum Mol Genet.2012 Aug 1;21(15): 3366-73. PMID: 22547223. Western Blot: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0. 03-0.1ug/ml. Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). See also publication Raya A. et al. , Nature.2009 Jul 2;460(7251): 53-9, PMID: 19483674.

other info1 :

Immunogen: Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2. Epitope: Internal region

ncbi gi num :

153945816

ncbi acc num :

NP_079141.2

ncbi gb acc num :

NM_024865.3

ncbi mol weight :

32,838 Da

ncbi pathways :

Cardiac Progenitor Differentiation Pathway (712094); Developmental Biology Pathway (1270302); POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway (1270347); POU5F1 (OCT4), SOX2, NANOG Repress Genes Related To Differentiation Pathway (1270348); Proteoglycans In Cancer Pathway (782000); Proteoglycans In Cancer Pathway (782054); SIDS Susceptibility Pathways (198901); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026136); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502); Transcriptional Regulation Of Pluripotent Stem Cells Pathway (1270346)

ncbi summary :

The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

uniprot summary :

NANOG: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell development/differentiation; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: nucleolus; nucleoplasm; nucleus. Molecular Function: DNA binding; protein binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity. Biological Process: cell differentiation; cell proliferation; endodermal cell fate specification; positive regulation of transcription from RNA polymerase II promoter; regulation of cell differentiation; regulation of gene expression; regulation of transcription, DNA-dependent; somatic stem cell maintenance; stem cell maintenance; transcription, DNA-dependent

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage