Goat anti-GAPDH (C Terminus) Loading Control Antibody | MyBioSource MBS420177 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-GAPDH (C Terminus) Loading Control Antibody

catalog :

MBS420177

quantity :

0.1 mg

price :

290 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420177

products type :

Antibody

products full name :

Goat anti-GAPDH (C Terminus) Loading Control Antibody

products short name :

GAPDHLoading Control

products name syn :

GAPDH; glyceraldehyde-3-phosphate dehydrogenase; HGNC:4141; G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432; aging-associated gene 9 protein; glyceraldehyde 3-phosphate dehydrogenase; Goat Anti-GAPDH (Internal); GAPDH; glyceraldehyde-3-phosphate dehydrogenase; HGNC:4141; G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432; aging-associated gene 9 protein; glyceraldehyde 3-phosphate dehydrogenase

other names :

glyceraldehyde-3-phosphate dehydrogenase isoform 1; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; epididymis secretory sperm binding protein Li 162eP; glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)

products gene name :

GAPDH

other gene names :

GAPDH; GAPDH; G3PD; GAPD; HEL-S-162eP; GAPD; GAPDH

uniprot entry name :

G3P_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Human, Mouse, Dog, Rat

sequence length :

335

sequence :

HQVVSSDFNSDT

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

app notes :

Immunohistochemistry: In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed. Recommended concentration 0.3ug/ml (data not shown). Western Blot: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037). The observed 35kDa was reduced upon siRNA-mediated GAPDH knock down expression. Recommended concentration: 0.01-0.03ug/ml. Peptide ELISA: antibody detection limit dilution 1:2000. Immunocytochemistry: PFA-fixed HeLa shows cytoplasm staining,dramatically reduced upon siRNA-mediated GAPDH knock down expression. Recommended concentration, 0.1-0.3ug/ml.

other info1 :

Epitope: C Terminus. Immunogen: Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2. Corresponding Immunizing Peptide: Catalog #MBS428417

other info2 :

Important Note: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.

products references :

Henson MA, Roberts AC, Salimi K, Vadlamudi S, Hamer RM, Gilmore JH, Jarskog LF, Philpot BD.; Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex.; Cereb Cortex. 2008 Nov; 18(11):2560-73.; Pubmed 18296432

ncbi gi num :

7669492

ncbi acc num :

NP_002037.2

ncbi gb acc num :

NM_002046.5

ncbi mol weight :

31,548 Da

ncbi pathways :

Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Alzheimers Disease Pathway 672448!!Biosynthesis Of Amino Acids Pathway 790012!!Biosynthesis Of Amino Acids Pathway 795174!!Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Disease Pathway 530764!!Gluconeogenesis Pathway 106204!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 413342

ncbi summary :

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

size :

0.1 mg

price :

290 USD

more info or order :

MyBioSource product webpage