antibody

Goat anti-WHIP / WRNIP1 Antibody

MBS420165

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-WHIP / WRNIP1 Antibody

WHIP/WRNIP1

WHIP; Werner helicase interacting protein; FLJ22526; putative helicase RUVBL; Werner helicase interacting protein 1; RP11-420G6.2; bA420G6.2; WHIP; Werner helicase interacting protein; FLJ22526; putative helicase RUVBL; Werner helicase interacting protein 1; RP11-420G6.2; bA420G6.2;

ATPase WRNIP1 isoform 1; ATPase WRNIP1; ATPase WRNIP1; putative helicase RUVBL; Werner helicase-interacting protein 1; Werner helicase interacting protein 1; Werner helicase-interacting protein 1

WRNIP1

WRNIP1; WRNIP1; WHIP; bA420G6.2

WRIP1_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Dog, Cow

665

EELRGVDFFKQRRC

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining, stronger in DCT compared to PCT. Recommended concentration, 4-6ug/ml. Western Blot: Approx 90kDa band observed in lysates of cell line Jurkat (calculated MW of 72.1kDa according to NP_064520.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bish RA, Myers MP., J Biol Chem. 2007 Aug 10;282(32):23184-93; PMID: 17550899). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide. Peptide ELISA: antibody detection limit dilution 1:32000.

Epitope: C Terminus. Immunogen: Peptide with sequence EELRGVDFFKQRRC, from the C Terminus of the protein sequence according to NP_064520.2; NP_569079.1. Corresponding Immunizing Peptide: Catalog #MBS426783

Important Note: This antibody is expected to recognise isoform 1 (NP_064520.2) and isoform 2 (NP_569079.1).

18426902

NP_064520.2

NM_020135.2

31,339 Da

Werner's syndrome is a rare autosomal recessive disorder characterized by accelerated aging that is caused by defects in the Werner syndrome ATP-dependent helicase gene (WRN). The protein encoded by this gene interacts with the exonuclease-containing N-terminal portion of the Werner protein. This protein has a ubiquitin-binding zinc-finger domain in the N-terminus, an ATPase domain, and two leucine zipper motifs in the C-terminus. It has sequence similarity to replication factor C family proteins and is conserved from E. coli to human. This protein likely accumulates at sites of DNA damage by interacting with polyubiquinated proteins and also binds to DNA polymerase delta and increases the initiation frequency of DNA polymerase delta-mediated DNA synthesis. This protein also interacts with nucleoporins at nuclear pore complexes. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq, Jul 2012]

0.1 mg

290 USD