Goat anti-p70S6K/RPS6KB1 Antibody | MyBioSource MBS420164 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-p70S6K/RPS6KB1 Antibody

catalog :

MBS420164

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420164

products type :

Antibody

products full name :

Goat anti-p70S6K/RPS6KB1 Antibody

products short name :

p70S6K/RPS6KB1

products name syn :

RPS6KB1; p70-S6K; ribosomal protein S6 kinase, 70kD, polypeptide 1; PS6K; S6K; S6K1; STK14A; p70(S6K)-alpha; p70-alpha; p70 S6 kinase, alpha 1; p70 S6 kinase, alpha 2; ribosomal protein S6 kinase, 70kD, polypeptide 1; serine/threonine kinase 14 alpha; RPS6KB1 antibody; p70-S6K antibody; ribosomal protein S6 kinase; 70kD; polypeptide 1 antibody; PS6K antibody; S6K antibody; S6K1 antibody; STK14A antibody; p70(S6K)-alpha antibody; p70-alpha antibody; p70 S6 kinase; alpha 1 antibody; p70 S6 kinase; alpha 2 antibody; ribosomal protein S6 kinase; 70kD; polypeptide 1 antibody; serine/threonine kinase 14 alpha antibody; p70S6K / RPS6KB1

other names :

ribosomal protein S6 kinase beta-1 isoform a; Ribosomal protein S6 kinase beta-1; ribosomal protein S6 kinase beta-1; ribosomal protein S6 kinase B1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA

products gene name :

RPS6KB1

products gene name syn :

p70S6K

other gene names :

RPS6KB1; RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha; STK14A; S6K-beta-1; S6K1; P70S6K1; p70-S6K 1; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA

uniprot entry name :

KS6B1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

sequence length :

525

sequence :

MISKRPEHLRMNL

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: In paraffin embedded Human Kidney shows staining in the cytoplasm-rich PCT while less so in the DCT and no signals in the glomerulus. Recommended concentration, 2-4ug/ml. Western Blot: Approx 65kDa band observed in lysates of Human Placenta, of Rat Brain and of cell line NIH3T3 (calculated MW of 59.1kDa according to NP_003152.1). Recommended concentration: 0.05-0.1ug/ml.

other info1 :

Immunogen: Peptide with sequence C-MISKRPEHLRMNL, from the C Terminus of the protein sequence according to NP_003152.1. Epitope: C Terminus

ncbi gi num :

4506737

ncbi acc num :

NP_003152.1

ncbi gb acc num :

NM_003161.3

ncbi mol weight :

51,016 Da

ncbi pathways :

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); B Cell Receptor Signaling Pathway (198909); BDNF Signaling Pathway (712093); CDC42 Signaling Events Pathway (137994); Choline Metabolism In Cancer Pathway (1059539)

ncbi summary :

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]

uniprot summary :

p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported. Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Translation; Protein kinase, AGC; AGC group; RSK family; p70 subfamily. Chromosomal Location of Human Ortholog: 17q23.1. Cellular Component: cell junction; cell surface; cytoplasm; cytosol; mitochondrial outer membrane; mitochondrion; neuron projection; nucleoplasm; nucleus; perinuclear region of cytoplasm; synapse. Molecular Function: ATP binding; identical protein binding; kinase activity; PDZ domain binding; peptide binding; protein binding; protein kinase activity; protein phosphatase 2A binding; protein serine/threonine/tyrosine kinase activity; ribosomal protein S6 kinase activity. Biological Process: aging; apoptosis; behavioral fear response; cell migration; cellular response to hormone stimulus; G1/S transition of mitotic cell cycle; germ cell development; long-term memory; negative regulation of apoptosis; negative regulation of insulin receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of mitotic cell cycle; positive regulation of skeletal muscle growth; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of translation; positive regulation of translational initiation; protein amino acid phosphorylation; protein kinase B signaling cascade; regulation of glucose import; response to drug; response to electrical stimulus involved in regulation of muscle adaptation; response to ethanol; response to glucagon stimulus; response to glucocorticoid stimulus; response to glucose stimulus; response to heat; response to insulin stimulus; response to leucine; response to lipopolysaccharide; response to mechanical stimulus; response to nutrient; response to testosterone stimulus; response to toxin; response to wounding; signal transduction; skeletal muscle atrophy; skeletal muscle contraction; TOR signaling pathway

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage