catalog number :
MBS420152
products full name :
Goat anti-SIAH1 Antibody
products short name :
SIAH1
products name syn :
SIAH1; seven in absentia homolog 1 (Drosophila); Siah-1; hSIAH1; HUMSIAH; Siah-1a; FLJ08065; seven in absentia homolog 1; sonic hedgehog homolog; SIAH1; seven in absentia homolog 1 (Drosophila); Siah-1; hSIAH1; HUMSIAH; Siah-1a; FLJ08065; seven in absentia homolog 1; sonic hedgehog homolog
other names :
E3 ubiquitin-protein ligase SIAH1 isoform a; E3 ubiquitin-protein ligase SIAH1; E3 ubiquitin-protein ligase SIAH1; siah-1a; seven in absentia homolog 1; siah E3 ubiquitin protein ligase 1; Seven in absentia homolog 1; Siah-1; Siah-1a
products gene name :
SIAH1
other gene names :
SIAH1; SIAH1; SIAH1A; HUMSIAH; Siah-1
uniprot entry name :
SIAH1_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Cow
sequence :
SRQTATALPTGTSKC
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
app notes :
Western Blot: Approx 28kDa band observed in Human Liver lysates (calculated MW of 31.1kDa according to NP_003022.3). Recommended concentration: 1-3ug/ml. An anonymous customer found positive results in WB on Human Jurkat cells. Peptide ELISA: antibody detection limit dilution 1:16000. Immunoprecipitation: This antibody has been successfully used in IP from CSM14.1 cells as described in the following paper: Liman et al, Molecules 2010, 15, 6678-6687.
other info1 :
Epitope: N Terminus. Immunogen: Peptide with sequence SRQTATALPTGTSKC, from the N Terminus of the protein sequence according to NP_003022.3; NP_001006611.1. Corresponding Immunizing Peptide: Catalog #MBS425579
other info2 :
Important Note: This product is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1)
ncbi acc num :
NP_003022.3
ncbi gb acc num :
NM_003031.3
ncbi mol weight :
21,215 Da
ncbi pathways :
Axon Guidance Pathway 105688!!Developmental Biology Pathway 477129!!Netrin-1 Signaling Pathway 160999!!Parkin-Ubiquitin Proteasomal System Pathway 700638!!Ubiquitin Mediated Proteolysis Pathway 83056!!Ubiquitin Mediated Proteolysis Pathway 466!!Wnt Signaling Pathway 83061!!Wnt Signaling Pathway 471!!P53 Signaling Pathway 83055!!P53 Signaling Pathway 465
ncbi summary :
This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]